Overview

  • Product name
    Endothelin 1 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Buffer 20 35.9pg/ml 6.7%
    Buffer 20 2.3pg/ml 8.9%
    Buffer 20 1.1pg/ml 8.8%
    Inter-assay
    Sample n Mean SD CV%
    Buffer 20 35.1pg/ml 8.3%
    Buffer 20 2.5pg/ml 5.9%
    Buffer 20 1.2pg/ml 15.3%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.41 pg/ml
  • Range
    0.78 pg/ml - 100 pg/ml
  • Assay time
    2h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s Endothelin 1 (ET-1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Endothelin 1 in serum, plasma and culture fluids. ET-1 has identical amino acid sequence for Human, mouse, rat, cow, dog, pig, and rabbit.

    Endothelin 1 specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated I Endothelin 1 detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the Endothelin 1 amount of sample captured in plate.

  • Notes

    Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ETB receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.

    Cross Reactivity

    CompoundCross Reactivity
    Endothelin 1 (ET-1)100
    ET-221
    ET-33.6
    Human Big ET-1<0.1
    Rat Big ET-1<0.1
    Human Big ET-2<0.1
    Human Big ET-3 Amide<0.1
  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Endothelin 1 measured in biological fluids of helathy species showing quantity (pg) per mL of tested sample. Results obtained from samples that were not extracted. Samples diluted 1-9 fold.

  • Representative Standard Curve using ab133030.

Protocols

References

This product has been referenced in:
  • Morsy MD  et al. The impact of concomitant administration of vanadium and insulin on endothelial dysfunction markers (PAI-1 and ET-1) in type 1 diabetic rats. Arch Physiol Biochem N/A:1-8 (2019). Read more (PubMed: 30789058) »
  • Liu ZY  et al. Less Vertebral Bone Mass after Treatment with Macitentan in Mice: A Pilot Study. Biomed Res Int 2019:2075968 (2019). Read more (PubMed: 30911541) »
See all 10 Publications for this product

Customer reviews and Q&As

Answer


The source of the Endothelin 1 ELISA kit informed us that :

"Extraction is only necessary if the samples have very low levels.
For samples like serum or plasma, it was found that the matrix interference could be diluted away by doing at least a 1:4 dilution or could be used without dilution if extracted.
Really, whether extraction is needed is dependent upon expected levels in the sample.
You can review general literature about expected levels and compare it to the kits sensitivity to see if they think it will be necessary."

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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