Key features and details
- Sensitivity: 0.41 pg/ml
- Range: 0.78 pg/ml - 100 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Pig
Product nameEndothelin 1 ELISA Kit
Intra-assay Sample n Mean SD CV% Buffer 20 35.9pg/ml 6.7% Buffer 20 2.3pg/ml 8.9% Buffer 20 1.1pg/ml 8.8% Inter-assay Sample n Mean SD CV% Buffer 20 35.1pg/ml 8.3% Buffer 20 2.5pg/ml 5.9% Buffer 20 1.2pg/ml 15.3%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.78 pg/ml - 100 pg/ml
Assay time2h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Pig
Endothelin 1 ELISA kit (ET-1) is designed for the accurate quantitative measurement of Endothelin 1 in serum, plasma and culture fluids. ET-1 has identical amino acid sequence for Human, mouse, rat, cow, dog, pig, and rabbit.
Endothelin 1 specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated I Endothelin 1 detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the Endothelin 1 amount of sample captured in plate.
Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ETB receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.
Compound Cross Reactivity Endothelin 1 (ET-1) 100 ET-2 21 ET-3 3.6 Human Big ET-1 <0.1 Rat Big ET-1 <0.1 Human Big ET-2 <0.1 Human Big ET-3 Amide <0.1
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 100ml Antibody Diluent 1 x 10ml Assay Buffer 17 1 x 100ml Endothelin 1 Antibody Concentrate 1 x 100µl Endothelin 1 Standard 1 x 0.25ml Mouse monoclonal Endothelin 1 Microplate (12 x 8 wells) 1 unit Plate Sealer 3 units Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml
RelevanceEndothelins are endothelium-derived vasoconstrictor peptides.
- Big endothelin 1
ab133030 has been referenced in 16 publications.
- Izumiya Y et al. Unusual morphologies of blood eosinophils in GM-CSF-producing lung cancer. QJM 114:42-44 (2021). PubMed: 32343814
- Ibrahimi Disha S et al. The role of endothelin B receptor in bone modelling during orthodontic tooth movement: a study on ETB knockout rats. Sci Rep 10:14226 (2020). PubMed: 32848199
- Wang H et al. Modulating effects of preconditioning exercise in the expression of ET-1 and BNP via HIF-1a in ischemically injured brain. Metab Brain Dis 34:1299-1311 (2019). PubMed: 31222402
- Wang Y et al. Potential role of sympathetic activity on the pathogenesis of massive pulmonary embolism with circulatory shock in rabbits. Respir Res 20:97 (2019). PubMed: 31118045
- Morsy MD et al. The impact of concomitant administration of vanadium and insulin on endothelial dysfunction markers (PAI-1 and ET-1) in type 1 diabetic rats. Arch Physiol Biochem N/A:1-8 (2019). PubMed: 30789058