Overview

  • Product name

    Anti-Endothelin 2/ET-2 antibody
    See all Endothelin 2/ET-2 primary antibodies
  • Description

    Rabbit polyclonal to Endothelin 2/ET-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Endothelin 2/ET-2 aa 1-178.
    Database link: P20800

  • Positive control

    • A549 whole cell lysate, HeLa whole cell lysate (ab150035)
  • General notes

     This product was previously labelled as Endothelin 2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 20 kDa.

Target

  • Function

    Endothelins are endothelium-derived vasoconstrictor peptides.
  • Tissue specificity

    Expressed in lung, but not in placental stem villi vessels or cultured placental villi smooth muscle cells.
  • Sequence similarities

    Belongs to the endothelin/sarafotoxin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • EDN2 antibody
    • EDN2_HUMAN antibody
    • Endothelin 2 antibody
    • Endothelin-2 antibody
    • ET 2 antibody
    • ET-2 antibody
    • ET2 antibody
    • PPET2 antibody
    • Preproendothelin 2 antibody
    • Preproendothelin-2 antibody
    see all

Images

  • Anti-Endothelin 2/ET-2 antibody (ab153867) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 20 kDa



    12% SDS PAGE

References

ab153867 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Purified protein (Ovary)
Gel Running Conditions
Reduced Denaturing (4-20% commercial Gel Reduced with DTT)
Loading amount
55 µg
Specification
Ovary
Blocking step
Commercial Blocking Solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 22 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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