Overview

  • Product name

    Anti-Endothelin 2/ET-2 antibody
    See all Endothelin 2/ET-2 primary antibodies
  • Description

    Rabbit polyclonal to Endothelin 2/ET-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein within Human Endothelin 2/ET-2 (internal sequence). The exact sequence is proprietary. Fusion protein corresponding to a region derived from internal residues of human Endothelin 2/ET-2 (BC034393). The protein fusion partner is GST.
    Database link: P20800

  • Positive control

    • Human cervical cancer tissue.
  • General notes

     This product was previously labelled as Endothelin 2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.

Target

  • Function

    Endothelins are endothelium-derived vasoconstrictor peptides.
  • Tissue specificity

    Expressed in lung, but not in placental stem villi vessels or cultured placental villi smooth muscle cells.
  • Sequence similarities

    Belongs to the endothelin/sarafotoxin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • EDN2 antibody
    • EDN2_HUMAN antibody
    • Endothelin 2 antibody
    • Endothelin-2 antibody
    • ET 2 antibody
    • ET-2 antibody
    • ET2 antibody
    • PPET2 antibody
    • Preproendothelin 2 antibody
    • Preproendothelin-2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Endothelin 2/ET-2 with ab197763 at 1/50 dilution.

References

This product has been referenced in:

  • Cacioppo JA  et al. Granulosa cell endothelin-2 expression is fundamental for ovulatory follicle rupture. Sci Rep 7:817 (2017). IHC . Read more (PubMed: 28400616) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Ovary)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate, pH 6.0, 110C for 30 min at 6 psi
Permeabilization
Yes - 0.1% triton in PBS for 10 minutes
Specification
Ovary
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Ms. Kirstin Cho

Verified customer

Submitted Feb 05 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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