Key features and details
- Rabbit polyclonal to Endothelin 2/ET-2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Endothelin 2/ET-2 antibody
See all Endothelin 2/ET-2 primary antibodies
DescriptionRabbit polyclonal to Endothelin 2/ET-2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: HEK-293T overexpressing Endothelin 2/ET-2 with a C-terminal myc-DDK tag, whole cell lysate. IHC-P: Human bone marrow tissue. ICC/IF: U-251 MG cells.
This product was previously labelled as Endothelin 2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 20 kDa.|
FunctionEndothelins are endothelium-derived vasoconstrictor peptides.
Tissue specificityExpressed in lung, but not in placental stem villi vessels or cultured placental villi smooth muscle cells.
Sequence similaritiesBelongs to the endothelin/sarafotoxin family.
- Information by UniProt
- EDN2 antibody
- EDN2_HUMAN antibody
- Endothelin 2 antibody
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Endothelin 2/ET-2 (green) using ab246962 at 4 μg/ml in ICC/IF.
Paraffin-embedded human bone marrow tissue stained for Endothelin 2/ET-2 using ab246962 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-Endothelin 2/ET-2 antibody (ab246962) at 0.4 mg/ml
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T overexpressing Endothelin 2/ET-2 with a C-terminal myc-DDK tag, whole cell lysate.
Predicted band size: 20 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246962 has not yet been referenced specifically in any publications.