Anti-Endothelin A Receptor/ET-A antibody (ab76259)
Key features and details
- Rabbit polyclonal to Endothelin A Receptor/ET-A
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Endothelin A Receptor/ET-A antibody
See all Endothelin A Receptor/ET-A primary antibodies -
Description
Rabbit polyclonal to Endothelin A Receptor/ET-A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 49 kDa.
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ICC/IF |
1/500 - 1/1000.
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IHC-P |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 49 kDa. |
ICC/IF
1/500 - 1/1000. |
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. -
Tissue specificity
Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1909 Human
- Entrez Gene: 13617 Mouse
- GenBank: NM_001957 Human
- GenBank: NM_174308 Human
- Omim: 131243 Human
- SwissProt: P25101 Human
- SwissProt: Q61614 Mouse
- Unigene: 183713 Human
see all -
Alternative names
- Ednra antibody
- EDNRA_HUMAN antibody
- Endothelin 1 receptor precursor antibody
see all
Images
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All lanes : Anti-Endothelin A Receptor/ET-A antibody (ab76259) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells
Lane 2 : Extracts from COS-7 cells plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa -
Immunofluorescence analysis of LOVO cells using ab76259 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide. -
ab76259 (4µg/ml) staining Endothelin A/ET-A receptor in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the endothelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab76259 has been referenced in 6 publications.
- Stölting M et al. Monitoring Endothelin-A Receptor Expression during the Progression of Atherosclerosis. Biomedicines 8:N/A (2020). PubMed: 33255872
- Ladd MR et al. The Development of Newborn Porcine Models for Evaluation of Tissue-Engineered Small Intestine. Tissue Eng Part C Methods 24:331-345 (2018). PubMed: 29638197
- Mücke MM et al. Targeting Mucosal Endothelin-A-Receptor Expression by Fluorescence Endoscopy is Feasible to Detect and Characterize Colitis-Associated Cancer in Mice. Inflamm Bowel Dis 24:111-122 (2017). PubMed: 29272493
- Lupp A et al. Reassessment of endothelin receptor A expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-8. Peptides 66:19-25 (2015). IHC-P ; Human . PubMed: 25708416
- Irani S et al. Expression profile of endothelin 1 and its receptor endothelin receptor A in papillary thyroid carcinoma and their correlations with clinicopathologic characteristics. Ann Diagn Pathol 18:43-8 (2014). IHC-P ; Human . PubMed: 24332749
- Laurberg JR et al. High expression of GEM and EDNRA is associated with metastasis and poor outcome in patients with advanced bladder cancer. BMC Cancer 14:638 (2014). WB, IHC-P ; Human . PubMed: 25175477