Anti-Endothelin A Receptor/ET-A antibody (ab117521)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-Endothelin A Receptor/ET-A antibody
See all Endothelin A Receptor/ET-A primary antibodies -
Description
Rabbit polyclonal to Endothelin A Receptor/ET-A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Mouse Endothelin A Receptor/ET-A aa 408-427 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:KNQEQNNHNTERSSHKDSMN
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Positive control
- Mouse dorsal root ganglia; Mouse heart tissue; Endothelin A Receptor/ET-A transfected cells; Mouse heart lysate
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General notes
We have received mixed feedback about mouse cross reactivity, some lot works and some doesn't so we no longer guarantee this species.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% Whole serum -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab117521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/5000. Predicted molecular weight: 49 kDa. | |
IHC-P | 1/2000. | |
ICC/IF | 1/5000. |
Target
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Function
Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. -
Tissue specificity
Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 281749 Cow
- Entrez Gene: 450187 Dog
- Entrez Gene: 1909 Human
- Entrez Gene: 13617 Mouse
- Entrez Gene: 397457 Pig
- GenBank: NM_001957 Human
- GenBank: NM_174308 Human
- Omim: 131243 Human
see all -
Alternative names
- Ednra antibody
- EDNRA_HUMAN antibody
- Endothelin 1 receptor precursor antibody
see all
Images
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ab117521, at 1/5000 dilution, staining Endothelin A Receptor/ET-A in HEK293 transfected with Endothelin A receptor cells by Immunocytochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody (ab117521)
ab117251, at 1/2000 dilution, staining Endothelin A Receptor/ET-A in the atrium of mouse heart by Immunohistochemistry.
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Anti-Endothelin A Receptor/ET-A antibody (ab117521) at 1/5000 dilution + Mouse heart lysate
Predicted band size: 49 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Im EJ et al. Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A. Nat Commun 10:1387 (2019). Read more (PubMed: 30918259) »
- Choy MK et al. Promoter interactome of human embryonic stem cell-derived cardiomyocytes connects GWAS regions to cardiac gene networks. Nat Commun 9:2526 (2018). Read more (PubMed: 29955040) »