Anti-Endothelin A Receptor/ET-A antibody (ab117521)

Thank you very much for your call today and for letting us know about the trouble with the staining results.

Please keep me updated about the results after trying the protocol alterations that we discussed. I look forward to hearing from you, and if there is anything else that we can do for you please let me know.

Have a nice day.

DISCOUNT CODE: XXXX
Expiration date: 2012-10-02

Thank you for calling yesterday. I am very pleased to hear that you would like to acceptmy offer and testthe anti-Endothelin A Receptor antibody (ab117521)with frozen tissue. This code above will give you: 1 freeprimary antibody or the value off anyproduct before the expiration date. To redeem this offer, please submit an Abreview for ab117521 with frozen tissue (IHC) and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site:

https://www.abcam.com/Abreviews.

Remember, as I said we publish both positive and negative Abreviews on our datasheets and this will not affect the discount so please submit the results of your tests whatever they may be. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions or concerns, please do not hesitate to contact me again.

Ilook forward to receiving your Abreview and wish you all the best with your research.

The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/collaborationdiscount

Thank you for your follow up email and I am sorry that I did not notice it until I had already sent your reply.

You are correct in thinking that the anti-Endothelin A Receptor antibody (ab85163) has been used successfully in staining frozen tissue sections, however, this has as yet only been performed in rat tissue. This does not mean that it will not work with the mouse tissue, simply that we have not tried it. The homology of the immunogen used (Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Rat Endothelin A Receptor, SwissProt reference P26684), shares very high homology (100%)with the mouse protein (SwissProt reference http://www.uniprot.org/uniprot/Q61614). We would therefore predict that the antibody would be able to detect the mouse protein. However as we have not tried it ourselves we are not able to guarantee this.

If you would be interested you would again be eligible for a testing discount if you wanted to try ab85163 in staining frozen sections of mouse tissue.

I hope this information has been of some help. IF you have any further questions please do not hesitate to contact us again.

Thank you for your call today and for letting us know about the trouble with ab117521.

As we discussed, I would recommend permeabilizing the cells with 0.1-0.2% Tween 20 instead of with Triton, and using a 1:500-1:1000 dilution of antibody. Please keep me updated about the results using these alterations.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help. Have a great weekend!

Thank you for contacting Abcam regarding our anti-Endothelin Receptor A antibodies. Regarding these antibodies, all of the information that is currently available can be found on the product datasheets on our website. I have summarized the answers to your questions below. a) All of these antibodies have been tested in WB and with human samples and are guaranteed to work. These antibodies have been tested in the following samples: ab76259 - LOVO, COS-7; ab84673 - HeLa, HepG2, MCF7; ab117521 - HEK293. Currently, no one has let us know that they have published with these antibodies, therefore no referencese are currently available. Our website will be updated should this information become available. Additionally, no specific protocol is recommended for these products. The recommended starting dilutions for WB are: ab76259 - 1ug/ml; ab84673 - 1ug/ml; ab117521 - 1:5000. b) Currently, we do not have a distributor, but sell directly to Greece. Orders do require prepayment by credit card. c) The pricing for each antibody is €370 plus €42 for shipping. While ab76259 and ab84673 are in stock (will ship overnight once payment is received), there is a lead time of 2-3 weeks after an order is placed for ab117521. d) Our antibodies are shipped on wet ice (4oC) FedEx overnight. e) We have not tested PC-3 or LNCaP cells, but if these cell lines do express ENDRA, then we expect the antibody to detect the protein in these samples. f) All of our products are covered by our Abpromise: https://www.abcam.com/index.html?pageconfig=abpromise This states that as long as you contact us within 6 months of purchase, products are guaranteed to work as stated on the datasheet. Products are eligible for a replacement or refund should they not work as specified. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.