Overview

  • Product name
    Anti-Endothelin A Receptor/ET-A antibody
    See all Endothelin A Receptor/ET-A primary antibodies
  • Description
    Rabbit polyclonal to Endothelin A Receptor/ET-A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Mouse Endothelin A Receptor/ET-A aa 408-427 (C terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    KNQEQNNHNTERSSHKDSMN

  • Positive control
    • Mouse dorsal root ganglia; Mouse heart tissue; Endothelin A Receptor transfected cells; Mouse heart lysate
  • General notes

    We have received mixed feedback about mouse cross reactivity, some lot works and some doesn't so we no longer guarantee this species.

Properties

Applications

Our Abpromise guarantee covers the use of ab117521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 49 kDa.
IHC-P 1/2000.
ICC/IF 1/5000.

Target

  • Function
    Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.
  • Tissue specificity
    Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ednra antibody
    • EDNRA_HUMAN antibody
    • Endothelin 1 receptor precursor antibody
    • Endothelin 1 specific receptor antibody
    • Endothelin A receptor antibody
    • Endothelin ET A receptor antibody
    • Endothelin receptor ET1 specific type antibody
    • Endothelin receptor subtype A antibody
    • Endothelin receptor type A antibody
    • Endothelin-1 receptor antibody
    • ET A antibody
    • ET-A antibody
    • ETA antibody
    • ETA R antibody
    • ETA-R antibody
    • ETAR antibody
    • ETRA antibody
    • G protein coupled receptor antibody
    • hET AR antibody
    • hET-AR antibody
    • MFDA antibody
    see all

Images

  • ab117521, at 1/5000 dilution, staining Endothelin A Receptor/ET-A in HEK293 transfected with Endothelin A receptor cells  by Immunocytochemistry.

  • ab117251, at 1/2000 dilution, staining Endothelin A Receptor/ET-A in the atrium of mouse heart by Immunohistochemistry.

  • Anti-Endothelin A Receptor/ET-A antibody (ab117521) at 1/5000 dilution + Mouse heart lysate

    Predicted band size: 49 kDa

References

This product has been referenced in:
  • Choy MK  et al. Promoter interactome of human embryonic stem cell-derived cardiomyocytes connects GWAS regions to cardiac gene networks. Nat Commun 9:2526 (2018). Read more (PubMed: 29955040) »
  • Por ED  et al. Repeat low-level blast exposure increases transient receptor potential vanilloid 1 (TRPV1) and endothelin-1 (ET-1) expression in the trigeminal ganglion. PLoS One 12:e0182102 (2017). Read more (PubMed: 28797041) »
See all 4 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cryopreserved embryonic cerebellar neurons (QBMcel)
Permeabilization
No
Specification
Cryopreserved embryonic cerebellar neurons (QBMcel
Fixative
paraformaldehyde with picric acid

Ms. Babben Tinner

Verified customer

Submitted Sep 29 2015

Question
Answer

Thank you very much for your call today and for letting us know about the trouble with the staining results.

Please keep me updated about the results after trying the protocol alterations that we discussed. I look forward to hearing from you, and if there is anything else that we can do for you please let me know.

Have a nice day.

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Answer

DISCOUNT CODE: XXXX
Expiration date: 2012-10-02

Thank you for calling yesterday. I am very pleased to hear that you would like to acceptmy offer and testthe anti-Endothelin A Receptor antibody (ab117521)with frozen tissue. This code above will give you: 1 freeprimary antibody or the value off anyproduct before the expiration date. To redeem this offer, please submit an Abreview for ab117521 with frozen tissue (IHC) and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site:

https://www.abcam.com/Abreviews.

Remember, as I said we publish both positive and negative Abreviews on our datasheets and this will not affect the discount so please submit the results of your tests whatever they may be. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions or concerns, please do not hesitate to contact me again.

Ilook forward to receiving your Abreview and wish you all the best with your research.

The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/collaborationdiscount

Read More

Answer

Thank you for your follow up email and I am sorry that I did not notice it until I had already sent your reply.

You are correct in thinking that the anti-Endothelin A Receptor antibody (ab85163) has been used successfully in staining frozen tissue sections, however, this has as yet only been performed in rat tissue. This does not mean that it will not work with the mouse tissue, simply that we have not tried it. The homology of the immunogen used (Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Rat Endothelin A Receptor, SwissProt reference P26684), shares very high homology (100%)with the mouse protein (SwissProt reference http://www.uniprot.org/uniprot/Q61614). We would therefore predict that the antibody would be able to detect the mouse protein. However as we have not tried it ourselves we are not able to guarantee this.

If you would be interested you would again be eligible for a testing discount if you wanted to try ab85163 in staining frozen sections of mouse tissue.

I hope this information has been of some help. IF you have any further questions please do not hesitate to contact us again.

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Question
Answer

Thank you for your call today and for letting us know about the trouble with ab117521.

As we discussed, I would recommend permeabilizing the cells with 0.1-0.2% Tween 20 instead of with Triton, and using a 1:500-1:1000 dilution of antibody. Please keep me updated about the results using these alterations.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you, and I'll be happy to help. Have a great weekend!

Read More

Question

I'm interested in your antibodies: a) ab 76259, b) ab 84673, and c) ab 117521 for the detection of the endothelin receptor A (ENDRA) with Western Blot in multiple myeloma cell lines. a) Firstly, I would very much appreciate any feedback from you (i.e protocols, bibliography) of use/applicability of these antibodies for the detection of ENDRA by Western blot analysis in human cancer cell lines and especially in human multiple myeloma cell lines, or in any other human cancer cell line of hemopoietic origin (i.e leukemia). b) Also, do you currently have a distributor in Greece, or would it have to be a UK order? c) I would like to know the pricing (price + shipping fee) and the currently availability of the above mentioned antibodies, as well as the estimated delivery time (working days) from order receipt. d) Also, I would like to comfirm the fact that the antibodies would be shipped on dry ice and I would much appreciate to provide me with the former users of these products in my country (i.e hospitals, universities etc.). e) Furthemore, is it possible to use the human prostate cancer cell lines PC-3 and LNCaP as positive controls for the detection of ENDRA by Western blot and if it is so I would be very grateful if you suggest me the corresponding bibliography. f) Lastly, I would like to know the policy regarding your guarantee of functionality of these products. Specifically, in the event of antibodies arriving in a non usable condition is there possibility of a refund or replacing with a new one? Thank you in advance,

Read More
Answer

Thank you for contacting Abcam regarding our anti-Endothelin Receptor A antibodies. Regarding these antibodies, all of the information that is currently available can be found on the product datasheets on our website. I have summarized the answers to your questions below. a) All of these antibodies have been tested in WB and with human samples and are guaranteed to work. These antibodies have been tested in the following samples: ab76259 - LOVO, COS-7; ab84673 - HeLa, HepG2, MCF7; ab117521 - HEK293. Currently, no one has let us know that they have published with these antibodies, therefore no referencese are currently available. Our website will be updated should this information become available. Additionally, no specific protocol is recommended for these products. The recommended starting dilutions for WB are: ab76259 - 1ug/ml; ab84673 - 1ug/ml; ab117521 - 1:5000. b) Currently, we do not have a distributor, but sell directly to Greece. Orders do require prepayment by credit card. c) The pricing for each antibody is €370 plus €42 for shipping. While ab76259 and ab84673 are in stock (will ship overnight once payment is received), there is a lead time of 2-3 weeks after an order is placed for ab117521. d) Our antibodies are shipped on wet ice (4oC) FedEx overnight. e) We have not tested PC-3 or LNCaP cells, but if these cell lines do express ENDRA, then we expect the antibody to detect the protein in these samples. f) All of our products are covered by our Abpromise: https://www.abcam.com/index.html?pageconfig=abpromise This states that as long as you contact us within 6 months of purchase, products are guaranteed to work as stated on the datasheet. Products are eligible for a replacement or refund should they not work as specified. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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