Overview

  • Product name

    Anti-Endothelin A Receptor/ET-A antibody
    See all Endothelin A Receptor/ET-A primary antibodies
  • Description

    Sheep polyclonal to Endothelin A Receptor/ET-A
  • Host species

    Sheep
  • Tested applications

    Suitable for: WB, ELISA, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Rat Endothelin A Receptor/ET-A aa 410-422 (C terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    QEQNHNTERSSHK

  • Positive control

    • Endothelin A Receptor/ET-A transfected COS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab30536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.
  • Tissue specificity

    Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ednra antibody
    • EDNRA_HUMAN antibody
    • Endothelin 1 receptor precursor antibody
    • Endothelin 1 specific receptor antibody
    • Endothelin A receptor antibody
    • Endothelin ET A receptor antibody
    • Endothelin receptor ET1 specific type antibody
    • Endothelin receptor subtype A antibody
    • Endothelin receptor type A antibody
    • Endothelin-1 receptor antibody
    • ET A antibody
    • ET-A antibody
    • ETA antibody
    • ETA R antibody
    • ETA-R antibody
    • ETAR antibody
    • ETRA antibody
    • G protein coupled receptor antibody
    • hET AR antibody
    • hET-AR antibody
    • MFDA antibody
    see all

References

This product has been referenced in:

  • Abed HS  et al. Obesity results in progressive atrial structural and electrical remodeling: implications for atrial fibrillation. Heart Rhythm 10:90-100 (2013). Read more (PubMed: 23063864) »
  • Riemekasten G  et al. Involvement of functional autoantibodies against vascular receptors in systemic sclerosis. Ann Rheum Dis 70:530-6 (2011). WB ; Human . Read more (PubMed: 21081526) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Q&A

Answer

Thank you for contacting us. At this time we do not offer the blocking peptide forthese products. However the immunogen sequences of both products are available on our website. You may be able to have custom peptide created from these for use as a blocking control. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your enquiry. Unfortunately, we do not have the IHC image available. However, the we do have the protocols as below. IHC-Paraffin (Tissue section: lung and intestine) Fixed paraffin-embedded sections (4-6 mm) are deparaffinized, stained, counterstained, dehydrated, cleared and mounted. Block the sections with 10% normal rabbit serum to inhibit non-specific binding. Dilute the antibody to 10ug/ml in PBS containing 1% normal rabbit serum. Incubate for 60min at RT or overnight at 4°C. Wash slides and detect by using an IgG (HRP) Pab Rabbit x Sheep secondary antibody. Antigen retrieval is typically not required with this antibody, but if necessary, sections are treated with EDTA, pH 8.0 and steamed for 20 min followed by cooling for 20 min. Optimal conditions are to be determined by the researcher. Note: This antibody recognizes epitopes on the cytoplasmic side of the receptor; the cell membrane must be made permeable to the antibody for immunochemical analysis. I hope this information will still be helpful.

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