For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Dear Sir,Madam, Many thanks for your reply. I am going to do IHC on Thyroid cancer (parrafin block tissues).My antibodies are Anti-Endothelin -1 (ab2786) and Anti-Endothelin A Receptor (ab76259). I would be very grateful if you suggest me both positive and negative control tissues. With best regards,
Asked on Dec 20 2011
Thank you for your message and for kindly providing the requested details. I am pleased to provide the following information: Endothelin -1 antibody ab2786 According to the image on the datasheet, this antibody has been successfully used in human bowel samples, and I can suggest this would be a suitable positive control. According to the SwissProt/Uniprot protein database site, it is also expressed in the lung and placental stem villi vessels and these would also be suitable positive controls. As endothelin 1 is found in the smooth muscle tissue of blood vessels, it will be found in most tissue types. therefore, it would be difficult to suggest a negative control. I can recommend a literature search to obtain further information. Endothelin A Receptor antibody ab76259 According to the image on the datasheet, this antibody has been successfully used in human cerebellum samples, and I can suggest this would be a suitable positive control. Its tissue specificity, from the SwissProt/Uniprot protein database link is as follows: Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels. Therefore, these tissues would also be suitable positive controls. Again, Endothelin A receptor it is quite widely expressed, and so suggesting a suitable negative control is difficult. I hope this information is helpful to you. Should you have any further questions, please do not hesitate to contact us.
Answered on Dec 20 2011