Overview

  • Product name

    Anti-Endothelin A Receptor/ET-A antibody
    See all Endothelin A Receptor/ET-A primary antibodies
  • Description

    Rabbit polyclonal to Endothelin A Receptor/ET-A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to Rat Endothelin A Receptor/ET-A aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab93498)

  • Positive control

    • This antibody gave a positive signal in the following rat tissue lysates: Brain; Cerebellum.

Properties

Applications

Our Abpromise guarantee covers the use of ab85163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa).

Target

  • Function

    Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.
  • Tissue specificity

    Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ednra antibody
    • EDNRA_HUMAN antibody
    • Endothelin 1 receptor precursor antibody
    • Endothelin 1 specific receptor antibody
    • Endothelin A receptor antibody
    • Endothelin ET A receptor antibody
    • Endothelin receptor ET1 specific type antibody
    • Endothelin receptor subtype A antibody
    • Endothelin receptor type A antibody
    • Endothelin-1 receptor antibody
    • ET A antibody
    • ET-A antibody
    • ETA antibody
    • ETA R antibody
    • ETA-R antibody
    • ETAR antibody
    • ETRA antibody
    • G protein coupled receptor antibody
    • hET AR antibody
    • hET-AR antibody
    • MFDA antibody
    see all

Images

  • All lanes : Anti-Endothelin A Receptor/ET-A antibody (ab85163) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    The band observed at 46 kDa could potentially be a cleaved form of Rat Endothelin-1 receptor precursor, as the target protein contains a 20 amino acid signal peptide, which when removed gives an expected molecular weight of 46 kDa.
  • All lanes : Anti-Endothelin A Receptor/ET-A antibody (ab85163) at 1/250 dilution (in 5% BSA + TBS + 0.1% Tween20 for 12 hours at 4°C)

    All lanes : Whole tissue lysate of rat lung

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/1000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa


    Exposure time: 30 seconds


    Blocking step: 5% Milk for 1 hour at 23°C

    See Abreview

References

This product has been referenced in:

  • Li L  et al. Downregulation of endothelin A receptor (ETaR) ameliorates renal ischemia reperfusion injury by increasing nitric oxide production. Life Sci 228:295-304 (2019). Read more (PubMed: 31075232) »
  • Silpanisong J  et al. Chronic hypoxia alters fetal cerebrovascular responses to endothelin-1. Am J Physiol Cell Physiol 313:C207-C218 (2017). Read more (PubMed: 28566491) »
See all 8 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Blood)
Permeabilization
No
Specification
Blood
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 13 2015

Answer

Thank you for your follow up email and I am sorry that I did not notice it until I had already sent your reply.

You are correct in thinking that the anti-Endothelin A Receptor antibody (ab85163) has been used successfully in staining frozen tissue sections, however, this has as yet only been performed in rat tissue. This does not mean that it will not work with the mouse tissue, simply that we have not tried it. The homology of the immunogen used (Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Rat Endothelin A Receptor, SwissProt reference P26684), shares very high homology (100%)with the mouse protein (SwissProt reference http://www.uniprot.org/uniprot/Q61614). We would therefore predict that the antibody would be able to detect the mouse protein. However as we have not tried it ourselves we are not able to guarantee this.

If you would be interested you would again be eligible for a testing discount if you wanted to try ab85163 in staining frozen sections of mouse tissue.

I hope this information has been of some help. IF you have any further questions please do not hesitate to contact us again.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (lung tissue)
Permeabilization
No
Specification
lung tissue
Blocking step
SuperBlock Blocking Buffer as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 23°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Dec 02 2010

Application
Western blot
Sample
Rat Tissue lysate - whole (rat (lung))
Loading amount
40 µg
Specification
rat (lung)
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Mr. Nico Jentsch

Verified customer

Submitted Jun 22 2010

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