RabMAb

Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

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Overview

  • Product name

    Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]
    See all Endothelin A Receptor/ET-A primary antibodies

  • Description

    Rabbit monoclonal [UMB-8-37-1] to Endothelin A Receptor/ET-A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide corresponding to Human Endothelin A Receptor/ET-A aa 400-500.
    Database link: P25101

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Mouse Heart.

  • General notes

    The epitope recognized by the UMB-8-37-1 monoclonal contains phosphorylation sites that affect signal in Western blot after treatment with endothelin. Phosphatase treatment may be required prior to Western blot in order to improve signal (PubMed ID 25708416).

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab178454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 60-90 kDa (predicted molecular weight: 49 kDa). PubMed: 25708416

The broad band size represents differentially glycosylated forms of the ETA receptor. Upon deglycosylation, a band at about 48 kDa is detected.
ICC/IF Use at an assay dependent concentration. PubMed: 25708416

Target

  • Function

    Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.

  • Tissue specificity

    Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Ednra antibody
    • EDNRA_HUMAN antibody
    • Endothelin 1 receptor precursor antibody
    • Endothelin 1 specific receptor antibody
    • Endothelin A receptor antibody
    • Endothelin ET A receptor antibody
    • Endothelin receptor ET1 specific type antibody
    • Endothelin receptor subtype A antibody
    • Endothelin receptor type A antibody
    • Endothelin-1 receptor antibody
    • ET A antibody
    • ET-A antibody
    • ETA antibody
    • ETA R antibody
    • ETA-R antibody
    • ETAR antibody
    • ETRA antibody
    • G protein coupled receptor antibody
    • hET AR antibody
    • hET-AR antibody
    • MFDA antibody
    see all

Images

  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)Lupp A et al., Peptides, 66, 19-25, Fig 1,; doi: 10.1016/j.peptides.2015.02.005. With permission from Elsevier

    Western blot analysis of stably ETA transfected HEK-293 cells after stimulation with ET-1 (1 μM) for 0, 5 or 10 min, respectively. ETA receptors were enriched using wheat germ lectin agarose beads and either not treated (−λPP) or subjected to enzymatic dephosphorylation using Lambda-phosphatase (+λPP). Blots were then incubated with ab178454 and developed using enhanced chemiluminescence.

    For more protocol information please see PMID: 25708416.

  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)Lupp A et al., Peptides, 66, 19-25, Fig 1,; doi: 10.1016/j.peptides.2015.02.005. With permission from Elsevier

    Western blot analysis of whole cell preparations from non- or stably ETA-transfected HEK-293 cells. ETA receptors were enriched using wheat germ lectin agarose beads. Membranes were incubated with ab178454 and blots were developed using enhanced chemiluminescence.

    For more protocol information please see PMID: 25708416.

  • Immunocytochemistry/ Immunofluorescence - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Immunocytochemistry/ Immunofluorescence - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)Lupp A et al., Peptides, 66, 19-25, Fig 1,; doi: 10.1016/j.peptides.2015.02.005. With permission from Elsevier

    Immunocytochemistry of stably ETA-transfected HEK-293 cells. Cells were exposed for 30 min to ET-1 (1 μM) (+) or left untreated (−) and subsequently fixed and immunofluorescently stained with ab178454.

    For more protocol information please see PMID: 25708416.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

    IHC image of Endothelin A Receptor/ET-A staining in Mouse Heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab178454, 10 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)Lupp A et al., Peptides, 66, 19-25, Fig 3,; doi: 10.1016/j.peptides.2015.02.005. With permission from Elsevier

    Immunohistochemical localization of ETA in human normal lymph node (I) and in neoplastic tissues (A–H) using ab178454. For more protocol information please see PMID: 25708416.

References

This product has been referenced in:

  • Cianfrocca R  et al. Blocking endothelin-1-receptor/ß-catenin circuit sensitizes to chemotherapy in colorectal cancer. Cell Death Differ 24:1811-1820 (2017). WB ; Human . Read more (PubMed: 28708138) »
  • Kaemmerer D  et al. Evaluation of somatostatin, CXCR4 chemokine and endothelin A receptor expression in a large set of paragangliomas. Oncotarget 8:89958-89969 (2017). IHC-P ; Human . Read more (PubMed: 29163802) »
See all 4 Publications for this product

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