RecombinantRabMAb

Recombinant Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

Submit a review Submit a question References (4)
Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
  • Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UMB-8-37-1] to Endothelin A Receptor/ET-A
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]
    See all Endothelin A Receptor/ET-A primary antibodies

  • Description

    Rabbit monoclonal [UMB-8-37-1] to Endothelin A Receptor/ET-A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Mouse heart and lung tissue lysates; IHC-P: Mouse and rat cardiac muscle and human placenta tissue.

  • General notes

    This product has switched from a hybridoma to recombinant production method on 9th September 2020.

    The epitope recognized by the UMB-8-37-1 monoclonal contains phosphorylation sites that affect signal in Western blot after treatment with endothelin. Phosphatase treatment may be required prior to Western blot in order to improve signal (PubMed ID 25708416).

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab178454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 40-70 kDa.

Target

  • Function

    Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.

  • Tissue specificity

    Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Ednra antibody
    • EDNRA_HUMAN antibody
    • Endothelin 1 receptor precursor antibody
    • Endothelin 1 specific receptor antibody
    • Endothelin A receptor antibody
    • Endothelin ET A receptor antibody
    • Endothelin receptor ET1 specific type antibody
    • Endothelin receptor subtype A antibody
    • Endothelin receptor type A antibody
    • Endothelin-1 receptor antibody
    • ET A antibody
    • ET-A antibody
    • ETA antibody
    • ETA R antibody
    • ETA-R antibody
    • ETAR antibody
    • ETRA antibody
    • G protein coupled receptor antibody
    • hET AR antibody
    • hET-AR antibody
    • MFDA antibody
    see all

Images

  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    All lanes : Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40-70 kDa
    Observed band size: 40-70 kDa


    Exposure time: 26 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed molecular weight is consistent with what has been described in the literature (PMID: 25708416).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

  • Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Western blot - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    All lanes : Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution

    Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293 transfected with Endothelin A Receptor/ET-A (WT) expression vector containing a myc-His-tag® , whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40-70 kDa
    Observed band size: 40-80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)Lupp A et al., Peptides, 66, 19-25, Fig 3,; doi: 10.1016/j.peptides.2015.02.005. With permission from Elsevier

    Immunohistochemical localization of ETA in human normal lymph node (I) and in neoplastic tissues (A–H) using ab178454. For more protocol information please see PMID: 25708416.

    Image generated from hybridoma version of ab178454

  • Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
    Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)

References (4)

Publishing research using ab178454? Please let us know so that we can cite the reference in this datasheet.

ab178454 has been referenced in 4 publications.

  • Cianfrocca R  et al. Blocking endothelin-1-receptor/ß-catenin circuit sensitizes to chemotherapy in colorectal cancer. Cell Death Differ 24:1811-1820 (2017). WB ; Human . PubMed: 28708138
  • Kaemmerer D  et al. Evaluation of somatostatin, CXCR4 chemokine and endothelin A receptor expression in a large set of paragangliomas. Oncotarget 8:89958-89969 (2017). IHC-P ; Human . PubMed: 29163802
  • Mahmoud AM  et al. Hyperinsulinemia augments endothelin-1 protein expression and impairs vasodilation of human skeletal muscle arterioles. Physiol Rep 4:N/A (2016). WB ; Human . PubMed: 27796268
  • Lupp A  et al. Reassessment of endothelin receptor A expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-8. Peptides 66:19-25 (2015). IHC-P, ICC, WB ; Mouse, Human . PubMed: 25708416

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