Anti-Endothelin B Receptor/ET-B antibody (ab117529)
Key features and details
- Rabbit polyclonal to Endothelin B Receptor/ET-B
- Suitable for: IHC-Fr, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Endothelin B Receptor/ET-B antibody
See all Endothelin B Receptor/ET-B primary antibodies -
Description
Rabbit polyclonal to Endothelin B Receptor/ET-B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, ICC/IF, IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Goat, Horse, Chicken, Cow, Dog, Pig, Rhesus monkey -
Immunogen
Synthetic peptide corresponding to Mouse Endothelin B Receptor/ET-B aa 400-500 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P48302 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr |
1/500 - 1/2000.
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ICC/IF | (2) |
1/5000.
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IHC-P | (1) |
1/2000.
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Notes |
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IHC-Fr
1/500 - 1/2000. |
ICC/IF
1/5000. |
IHC-P
1/2000. |
Target
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Function
Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Tissue specificity
Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells. -
Involvement in disease
Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily. -
Post-translational
modificationsPalmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 408082 Chicken
- Entrez Gene: 281750 Cow
- Entrez Gene: 100033875 Horse
- Entrez Gene: 1910 Human
- Entrez Gene: 13618 Mouse
- Entrez Gene: 100009477 Rabbit
- Omim: 131244 Human
- SwissProt: P28088 Cow
see all -
Alternative names
- ABCDS antibody
- Ednra antibody
- EDNRB antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor/ET-B with ab117529.
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Immunohistochemistry (Frozen sections) analysis of mouse skin tissue sections labeling Endothelin B Receptor/ET-B with ab117529 at 1/800 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor/ET-B with ab117529 at 1/1500 dilution. Tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C. The tissue was incubated with Anti-Endothelin B Receptor antibody (ab117529) for 12 hours in PBS at 20°C. A biotin conjugated goat anti-rabbit secondary antibody at 1/200 dilution.
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ab117529, at 1/5000 dilution, staining Endothelin B Receptor/ET-B in ETB-transfected HEK293 cells by Immunocytochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (35)
ab117529 has been referenced in 35 publications.
- Shi CJ et al. TGFβR-1/ALK5 inhibitor RepSox induces enteric glia-to-neuron transition and influences gastrointestinal mobility in adult mice. Acta Pharmacol Sin 44:92-104 (2023). PubMed: 35794374
- Bonnamour G et al. NR2F1 regulates a Schwann cell precursor-vs-melanocyte cell fate switch in a mouse model of Waardenburg syndrome type IV. Pigment Cell Melanoma Res 35:506-516 (2022). PubMed: 35816394
- Weigert A et al. Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system. Nat Commun 13:6078 (2022). PubMed: 36241617
- Zhang Y et al. Endothelin-1, over-expressed in SOD1G93A mice, aggravates injury of NSC34-hSOD1G93A cells through complicated molecular mechanism revealed by quantitative proteomics analysis. Front Cell Neurosci 16:1069617 (2022). PubMed: 36531135
- Del Rio D et al. Ovarian Cancer-Driven Mesothelial-to-Mesenchymal Transition is Triggered by the Endothelin-1/β-arr1 Axis. Front Cell Dev Biol 9:764375 (2021). PubMed: 34926453