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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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Anti-Endothelin B Receptor/ET-B antibody (ab117529)

  • Datasheet
  • SDS
Reviews (4)Q&A (4)References (20)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)
  • Immunohistochemistry (Frozen sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)
  • Immunocytochemistry - Anti-Endothelin B Receptor/ET-B antibody (ab117529)
  • Western blot - Anti-Endothelin B Receptor/ET-B antibody (ab117529)

Key features and details

  • Rabbit polyclonal to Endothelin B Receptor/ET-B
  • Suitable for: ICC, IHC-Fr, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Endothelin B Receptor/ET-B antibody
    See all Endothelin B Receptor/ET-B primary antibodies
  • Description

    Rabbit polyclonal to Endothelin B Receptor/ET-B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Goat, Horse, Chicken, Cow, Dog, Pig, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse Endothelin B Receptor/ET-B aa 400-500 (C terminal) conjugated to keyhole limpet haemocyanin.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Endothelin B Receptor/ET-B protein (ab152358)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab117529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/5000.
IHC-Fr
1/500 - 1/2000.
WB
1/5000. Predicted molecular weight: 50 kDa.
IHC-P (1)
1/2000.
Notes
ICC
1/5000.
IHC-Fr
1/500 - 1/2000.
WB
1/5000. Predicted molecular weight: 50 kDa.
IHC-P
1/2000.

Target

  • Function

    Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
  • Involvement in disease

    Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
    Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
  • Post-translational
    modifications

    Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P24530 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 408082 Chicken
    • Entrez Gene: 281750 Cow
    • Entrez Gene: 100033875 Horse
    • Entrez Gene: 1910 Human
    • Entrez Gene: 13618 Mouse
    • Entrez Gene: 100009477 Rabbit
    • Omim: 131244 Human
    • SwissProt: P28088 Cow
    • SwissProt: O62709 Horse
    • SwissProt: P24530 Human
    • SwissProt: P48302 Mouse
    • SwissProt: P35463 Pig
    • SwissProt: Q9N0W7 Rabbit
    • Unigene: 82002 Human
    • Unigene: 229532 Mouse
    see all
  • Alternative names

    • ABCDS antibody
    • Ednra antibody
    • EDNRB antibody
    • EDNRB_HUMAN antibody
    • Endothelin B receptor antibody
    • Endothelin B receptor precursor antibody
    • Endothelin receptor Non selective type antibody
    • Endothelin receptor non-selective type antibody
    • Endothelin receptor type B antibody
    • ET B antibody
    • ET-B antibody
    • ET-BR antibody
    • ETB antibody
    • ETBR antibody
    • ETRB antibody
    • Hirschsprung disease 2 antibody
    • HSCR antibody
    • HSCR2 antibody
    • OTTHUMP00000018534 antibody
    • OTTHUMP00000178736 antibody
    • WS4A antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor/ET-B with ab117529.

  • Immunohistochemistry (Frozen sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)
    Immunohistochemistry (Frozen sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)

    Immunohistochemistry (Frozen sections) analysis of mouse skin tissue sections labeling Endothelin B Receptor/ET-B with ab117529 at 1/800 dilution. Tissues were fixed for 10 minutes, blocked in 3% fish gelatin for 10-20 minutes, and probed with an Isotype Control Antibody (left panel) (1/800 dilution), and Anti-Endothelin B Receptor antibody (ab117529) (right panel) overnight at 4ºC. Detection was performed using a PE-conjugated anti-rabbit IgG secondary antibody at a dilution of 1/800 (red). Nuclei were stained with DAPI and tissues were visualized by fluorescence microscopy.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)This image is courtesy of an anonymous abreview.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor/ET-B with ab117529 at 1/1500 dilution. Tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C. The tissue was incubated with Anti-Endothelin B Receptor antibody (ab117529) for 12 hours in PBS at 20°C. A biotin conjugated goat anti-rabbit secondary antibody at 1/200 dilution.

    See Abreview

  • Immunocytochemistry - Anti-Endothelin B Receptor/ET-B antibody (ab117529)
    Immunocytochemistry - Anti-Endothelin B Receptor/ET-B antibody (ab117529)

    ab117529, at 1/5000 dilution, staining Endothelin B Receptor/ET-B in HEK293 cells by Immunocytochemistry.

  • Western blot - Anti-Endothelin B Receptor/ET-B antibody (ab117529)
    Western blot - Anti-Endothelin B Receptor/ET-B antibody (ab117529)
    Anti-Endothelin B Receptor/ET-B antibody (ab117529) at 1/5000 dilution + Mouse brain lysate

    Predicted band size: 50 kDa

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (20)

Publishing research using ab117529? Please let us know so that we can cite the reference in this datasheet.

ab117529 has been referenced in 20 publications.

  • Gupta S  et al. Irreversible and sustained upregulation of endothelin axis during oncogene-associated pancreatic inflammation and cancer. Neoplasia 22:98-110 (2020). PubMed: 31923844
  • Su E  et al. Aggravated endothelial endocrine dysfunction and intimal thickening of renal artery in high-fat diet-induced obese pigs following renal denervation. BMC Cardiovasc Disord 20:176 (2020). PubMed: 32295540
  • Halaka M  et al. Differences in Endothelin B Receptor Isoforms Expression and Function in Breast Cancer Cells. J Cancer 11:2688-2701 (2020). PubMed: 32201539
  • Adams KL  et al. Endothelin-1 signaling maintains glial progenitor proliferation in the postnatal subventricular zone. Nat Commun 11:2138 (2020). PubMed: 32358570
  • Jensen MF  et al. Endothelin-1 induces a strong pressor effect in ball pythons (Python regius). Comp Biochem Physiol A Mol Integr Physiol 241:110620 (2020). PubMed: 31770594
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-8 of 8 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Endothelin B Receptor/ET-B antibody

Good
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Skin tissue)
Permeabilization
Yes - 0.1% triton X-100
Specification
Skin tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Laura Grisanti

Verified customer

Submitted Oct 30 2019

Flow Cytometry abreview for Anti-Endothelin B Receptor/ET-B antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Rat Cell (Brain)
Permeabilization
Yes - 0.25 % Triton X-100
Gating Strategy
Viable cells
Specification
Brain
Fixation
Paraformaldehyde
Read More

Ms. Aneta Radziwon-Balicka

Verified customer

Submitted Oct 30 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Endothelin B Receptor/ET-B antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Blood)
Permeabilization
No
Specification
Blood
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 37°C
Fixative
life cell staining
Read More

Abcam user community

Verified customer

Submitted Oct 13 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Endothelin B Receptor/ET-B antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 05 2013

Question

Dear xxxxx,
Thank you for answering my question, so just to clarify would you suggest that I assume the concentration of the antibody is 10mg/ml or 1mg/ml when calculating the concentration/amount of the isotype control I use? I also wondered if you would recommend using an antigen retrieval method for this antibody and which one is best to use, eg citrate buffer or an enzymatic method?
Best Wishes

Read More

Abcam community

Verified customer

Asked on Oct 12 2012

Answer

I am sorry for the delay in getting back to you.
I now have confirmation that the antigen retrieval method we have used with the anti-Endothelin B Receptor antibody (ab117529) to obtain the image presented on the datasheet was citric acid buffer (pH 6) for 20 minutes in the microwave. This may require further optimisation to obtain the best results in your particular tissues.
I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

Read More

Abcam Scientific Support

Answered on Oct 12 2012

Question

Product code: 117529
Lot number: GR83314-3

Inquiry: Dear Sir/Madam, I have a query about what is the protein concentration of this antibody as I would like to use a rabbit IgG serum as a negative control alongside the antibody and I'm not sure what the actual concentration of the antibody is as it is not mentioned anywhere on the datasheet. Best Wishes

Read More

Abcam community

Verified customer

Asked on Oct 09 2012

Answer

Thank you for contacting us and your interest in our products.
Our Endothelin Receptor B Polyclonal Antibody - product ab117529 is provided as undiluted antisera. This contains many other proteins other than the specific antibodies and quantification of the concentration very difficult. We do not accurately quantify the immunoglobulin concentration but we approximate it at about 10mg/ml (of which about 10% of this is specific for Endothelin Receptor B, therefore ˜ 1mg/ml specific antibody).
For an isotype control we would recommend using the same amount/concentration of isotype control as your test antibody.
I hope this information is helpful. Please feel free to contact me again with any additional questions you may have.

Read More

Abcam Scientific Support

Answered on Oct 09 2012

Question

hiermit bestätige ich, dass ich den ab117529 Anti-Endothelin B Receptor antibodyin ICH-Fr testen möchte.

Der Antikörper wird zentral von unserem Department bestellt.

Read More

Abcam community

Verified customer

Asked on May 04 2012

Answer

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab84182 Anti-Endothelin B Receptor antibody - C-terminal in IHC-Fr zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer XXXXX, deckt den Wert von ab84182, ab und kann zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in IHC-Fr eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

Read More

Abcam Scientific Support

Answered on May 04 2012

Question

mouse, human, IHC-Fr

Read More

Abcam community

Verified customer

Asked on May 02 2012

Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde ab117529 Anti-Endothelin B Receptor antibody bisher noch nicht inIHC-Fr getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab117529abdecken, und gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab117529in IHC-Fr testen möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper in IHC-Fr.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderen primären Antikörpers zu dem gleichen Preis wie ab117529bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab117529bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

Read More

Abcam Scientific Support

Answered on May 02 2012

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