Overview

  • Product name
    Anti-Endothelin B Receptor/ET-B antibody
    See all Endothelin B Receptor/ET-B primary antibodies
  • Description
    Rabbit polyclonal to Endothelin B Receptor/ET-B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC, IHC-Fr, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Goat, Horse, Chicken, Cow, Dog, Pig, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse Endothelin B Receptor/ET-B aa 424-442 (C terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    KANDHGYDNFRSSNKYSSS

  • Positive control
    • Mouse lung tissue; Mouse brain lysate; Endothelin B Receptor transfected cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/5000.
IHC-Fr 1/500 - 1/2000.
WB 1/5000. Predicted molecular weight: 50 kDa.
IHC-P 1/2000.
ICC/IF 1/5000.

Target

  • Function
    Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity
    Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
  • Involvement in disease
    Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
    Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
  • Post-translational
    modifications
    Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABCDS antibody
    • Ednra antibody
    • EDNRB antibody
    • EDNRB_HUMAN antibody
    • Endothelin B receptor antibody
    • Endothelin B receptor precursor antibody
    • Endothelin receptor Non selective type antibody
    • Endothelin receptor non-selective type antibody
    • Endothelin receptor type B antibody
    • ET B antibody
    • ET-B antibody
    • ET-BR antibody
    • ETB antibody
    • ETBR antibody
    • ETRB antibody
    • Hirschsprung disease 2 antibody
    • HSCR antibody
    • HSCR2 antibody
    • OTTHUMP00000018534 antibody
    • OTTHUMP00000178736 antibody
    • WS4A antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor/ET-B with ab117529.

  • Immunohistochemistry (Frozen sections) analysis of mouse skin tissue sections labeling Endothelin B Receptor/ET-B with ab117529 at 1/800 dilution. Tissues were fixed for 10 minutes, blocked in 3% fish gelatin for 10-20 minutes, and probed with an Isotype Control Antibody (left panel) (1/800 dilution), and Anti-Endothelin B Receptor antibody (ab117529) (right panel) overnight at 4ºC. Detection was performed using a PE-conjugated anti-rabbit IgG secondary antibody at a dilution of 1/800 (red). Nuclei were stained with DAPI and tissues were visualized by fluorescence microscopy.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor/ET-B with ab117529 at 1/1500 dilution. Tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C. The tissue was incubated with Anti-Endothelin B Receptor antibody (ab117529) for 12 hours in PBS at 20°C. A biotin conjugated goat anti-rabbit secondary antibody at 1/200 dilution.

    See Abreview

  • ab117529, at 1/5000 dilution, staining Endothelin B Receptor/ET-B in HEK293 cells by Immunocytochemistry.

  • Anti-Endothelin B Receptor/ET-B antibody (ab117529) at 1/5000 dilution + Mouse brain lysate

    Predicted band size: 50 kDa

References

This product has been referenced in:
  • Liu W  et al. Gli family zinc finger 1 is associated with endothelin receptor type B in Hirschsprung disease. Mol Med Rep 17:5844-5850 (2018). Read more (PubMed: 29484400) »
  • Valentin-Torres A  et al. Blockade of sustained tumor necrosis factor in a transgenic model of progressive autoimmune encephalomyelitis limits oligodendrocyte apoptosis and promotes oligodendrocyte maturation. J Neuroinflammation 15:121 (2018). Read more (PubMed: 29690885) »
See all 6 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Flow Cytometry
Sample
Rat Cell (Brain)
Permeabilization
Yes - 0.25 % Triton X-100
Gating Strategy
Viable cells
Specification
Brain
Fixation
Paraformaldehyde

Ms. Aneta Radziwon-Balicka

Verified customer

Submitted Oct 30 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Blood)
Permeabilization
No
Specification
Blood
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 37°C
Fixative
life cell staining

Abcam user community

Verified customer

Submitted Oct 13 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 05 2013

Answer

I am sorry for the delay in getting back to you.
I now have confirmation that the antigen retrieval method we have used with the anti-Endothelin B Receptor antibody (ab117529) to obtain the image presented on the datasheet was citric acid buffer (pH 6) for 20 minutes in the microwave. This may require further optimisation to obtain the best results in your particular tissues.
I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

Read More

Answer

Thank you for contacting us and your interest in our products.
Our Endothelin Receptor B Polyclonal Antibody - product ab117529 is provided as undiluted antisera. This contains many other proteins other than the specific antibodies and quantification of the concentration very difficult. We do not accurately quantify the immunoglobulin concentration but we approximate it at about 10mg/ml (of which about 10% of this is specific for Endothelin Receptor B, therefore ˜ 1mg/ml specific antibody).
For an isotype control we would recommend using the same amount/concentration of isotype control as your test antibody.
I hope this information is helpful. Please feel free to contact me again with any additional questions you may have.

Read More

Answer

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab84182 Anti-Endothelin B Receptor antibody - C-terminal in IHC-Fr zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer XXXXX, deckt den Wert von ab84182, ab und kann zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in IHC-Fr eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

Read More

Question
Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde ab117529 Anti-Endothelin B Receptor antibody bisher noch nicht inIHC-Fr getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab117529abdecken, und gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab117529in IHC-Fr testen möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper in IHC-Fr.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderen primären Antikörpers zu dem gleichen Preis wie ab117529bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab117529bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up