Anti-Endothelin B Receptor/ET-B antibody (ab117529)
- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-Endothelin B Receptor/ET-B antibody
See all Endothelin B Receptor/ET-B primary antibodies -
DescriptionRabbit polyclonal to Endothelin B Receptor/ET-B
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Host speciesRabbit
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Tested applicationsSuitable for: ICC, IHC-Fr, WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Goat, Horse, Chicken, Cow, Dog, Pig, Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Mouse Endothelin B Receptor/ET-B aa 424-442 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:KANDHGYDNFRSSNKYSSS
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Positive control
- Mouse lung tissue; Mouse brain lysate; Endothelin B Receptor transfected cells
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% Whole serum -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab117529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/5000. | |
| IHC-Fr | 1/500 - 1/2000. | |
| WB | 1/5000. Predicted molecular weight: 50 kDa. | |
| IHC-P | 1/2000. | |
| ICC/IF | 1/5000. |
Target
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FunctionNon-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
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Tissue specificityExpressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
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Involvement in diseaseDefects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine. -
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
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Post-translational
modificationsPalmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405. -
Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 408082 Chicken
- Entrez Gene: 281750 Cow
- Entrez Gene: 100033875 Horse
- Entrez Gene: 1910 Human
- Entrez Gene: 13618 Mouse
- Entrez Gene: 100009477 Rabbit
- Omim: 131244 Human
- SwissProt: P28088 Cow
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Alternative names
- ABCDS antibody
- Ednra antibody
- EDNRB antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor/ET-B with ab117529.
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Immunohistochemistry (Frozen sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)Immunohistochemistry (Frozen sections) analysis of mouse skin tissue sections labeling Endothelin B Receptor/ET-B with ab117529 at 1/800 dilution. Tissues were fixed for 10 minutes, blocked in 3% fish gelatin for 10-20 minutes, and probed with an Isotype Control Antibody (left panel) (1/800 dilution), and Anti-Endothelin B Receptor antibody (ab117529) (right panel) overnight at 4ºC. Detection was performed using a PE-conjugated anti-rabbit IgG secondary antibody at a dilution of 1/800 (red). Nuclei were stained with DAPI and tissues were visualized by fluorescence microscopy.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)This image is courtesy of an anonymous abreview.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor/ET-B with ab117529 at 1/1500 dilution. Tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C. The tissue was incubated with Anti-Endothelin B Receptor antibody (ab117529) for 12 hours in PBS at 20°C. A biotin conjugated goat anti-rabbit secondary antibody at 1/200 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Endothelin B Receptor/ET-B antibody (ab117529)ab117529, at 1/5000 dilution, staining Endothelin B Receptor/ET-B in HEK293 cells by Immunocytochemistry.
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Western blot - Anti-Endothelin B Receptor/ET-B antibody (ab117529)Anti-Endothelin B Receptor/ET-B antibody (ab117529) at 1/5000 dilution + Mouse brain lysate
Predicted band size: 50 kDa
Protocols
References
This product has been referenced in:
- Liu W et al. Gli family zinc finger 1 is associated with endothelin receptor type B in Hirschsprung disease. Mol Med Rep 17:5844-5850 (2018). Read more (PubMed: 29484400) »
- Valentin-Torres A et al. Blockade of sustained tumor necrosis factor in a transgenic model of progressive autoimmune encephalomyelitis limits oligodendrocyte apoptosis and promotes oligodendrocyte maturation. J Neuroinflammation 15:121 (2018). Read more (PubMed: 29690885) »
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