Overview

  • Product name

    Anti-Endothelin B Receptor/ET-B antibody
    See all Endothelin B Receptor/ET-B primary antibodies
  • Description

    Rabbit polyclonal to Endothelin B Receptor/ET-B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Endothelin B Receptor/ET-B aa 413-442 (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Mouse cerebellum tissue lysate; NCI-H460 and 293 cells; Human prostate carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Purification notes

    ab135611 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab135611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 50 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/10 - 1/50.

Target

  • Function

    Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
  • Involvement in disease

    Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
    Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
  • Post-translational
    modifications

    Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABCDS antibody
    • Ednra antibody
    • EDNRB antibody
    • EDNRB_HUMAN antibody
    • Endothelin B receptor antibody
    • Endothelin B receptor precursor antibody
    • Endothelin receptor Non selective type antibody
    • Endothelin receptor non-selective type antibody
    • Endothelin receptor type B antibody
    • ET B antibody
    • ET-B antibody
    • ET-BR antibody
    • ETB antibody
    • ETBR antibody
    • ETRB antibody
    • Hirschsprung disease 2 antibody
    • HSCR antibody
    • HSCR2 antibody
    • OTTHUMP00000018534 antibody
    • OTTHUMP00000178736 antibody
    • WS4A antibody
    see all

Images

  • Anti-Endothelin B Receptor/ET-B antibody (ab135611) at 1/50 dilution + Mouse cerebellum tissue lysate at 35 µg

    Predicted band size: 50 kDa

  • Flow Cytometric analysis of NCI-H460 cells labelling Endothelin B Receptor/ET-B with ab135611 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunofluorescent analysis of 293 cells labelling Endothelin B Receptor/ET-B with ab135611 at 1/10 dilution followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labelling Endothelin B Receptor/ET-B with ab135611 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

References

ab135611 has not yet been referenced specifically in any publications.

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