Anti-Endothelin B Receptor/ET-B antibody (ab262693)
Key features and details
- Rabbit polyclonal to Endothelin B Receptor/ET-B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Endothelin B Receptor/ET-B antibody
See all Endothelin B Receptor/ET-B primary antibodies -
Description
Rabbit polyclonal to Endothelin B Receptor/ET-B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Endothelin B Receptor/ET-B (N terminal). The exact sequence is proprietary. Carrier protein conjugated.
Database link: P24530 -
Positive control
- WB: A431 and H1299 whole cell lysate. IHC-P: Human colon carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 49.99% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab262693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
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IHC-P |
1/100 - 1/1000.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa. |
IHC-P
1/100 - 1/1000. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
Target
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Function
Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Tissue specificity
Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells. -
Involvement in disease
Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily. -
Post-translational
modificationsPalmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1910 Human
- Omim: 131244 Human
- SwissProt: P24530 Human
- Unigene: 82002 Human
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Alternative names
- ABCDS antibody
- Ednra antibody
- EDNRB antibody
see all
Images
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Paraffin-embedded, formalin-fixed human colon carcinoma tissue stained for Endothelin B Receptor/ET-B using ab262693 at 1/250 dilution in immunohistochemical analysis.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15mins.
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All lanes : Anti-Endothelin B Receptor/ET-B antibody (ab262693) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa10% SDS-PAGE.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab262693 has not yet been referenced specifically in any publications.