Product nameAnti-Endothelin B Receptor/ET-B antibody
See all Endothelin B Receptor/ET-B primary antibodies
DescriptionRabbit polyclonal to Endothelin B Receptor/ET-B
Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Dog
- This antibody gave a positive result in the following lysates: Liver (Human) Membrane Lysate - adult normal tissue Placenta (Human) Tissue Lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab39960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21203549|
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).|
FunctionNon-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Tissue specificityExpressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
Involvement in diseaseDefects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
modificationsPalmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
Cellular localizationCell membrane.
- Information by UniProt
- ABCDS antibody
- Ednra antibody
- EDNRB antibody
ICC/IF image of ab39960 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39960, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-Endothelin B Receptor/ET-B antibody (ab39960) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ab39960 staining human placenta sections in IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed. Blocking of the sample was done with 5 minutes of peroxidase block and 10 minutes of protein block, followed by staining with ab39960 at 1/200 for 45 minutes at 20°C. A HRP conjugated goat anti-rabbit polyclonal antibody was used as the secondary antibody.
This product has been referenced in:
- Cianfrocca R et al. Blocking endothelin-1-receptor/ß-catenin circuit sensitizes to chemotherapy in colorectal cancer. Cell Death Differ 24:1811-1820 (2017). WB . Read more (PubMed: 28708138) »
- Hall SM et al. Endothelin receptor expression in idiopathic pulmonary arterial hypertension: effect of bosentan and epoprostenol treatment. Eur Respir J 38:851-60 (2011). WB ; Human . Read more (PubMed: 21406517) »