Recombinant Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7013] to Endothelin B Receptor/ET-B
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Endothelin B Receptor/ET-B antibody [EPR7013]
See all Endothelin B Receptor/ET-B primary antibodies -
Description
Rabbit monoclonal [EPR7013] to Endothelin B Receptor/ET-B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- Raji, HeLa, JAR, and 293T cell lysates; HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.21 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7013 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
IP
1/10 - 1/100. |
Target
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Function
Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Tissue specificity
Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells. -
Involvement in disease
Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily. -
Post-translational
modificationsPalmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1910 Human
- Omim: 131244 Human
- SwissProt: P24530 Human
- Unigene: 82002 Human
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Alternative names
- ABCDS antibody
- Ednra antibody
- EDNRB antibody
see all
Images
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All lanes : Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102) at 1/10000 dilution (purified)
Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
ab129102 (purified) at 1/40 immunoprecipitating Endothelin B receptor//ET-B in 10 μg 293T (Lanes 1 and 2, observed at 50 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102) at 1/1000 dilution (unpurified)
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : JAR cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 50 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab129102 has been referenced in 3 publications.
- Gibson DA et al. Intracrine Androgens Enhance Decidualization and Modulate Expression of Human Endometrial Receptivity Genes. Sci Rep 6:19970 (2016). PubMed: 26817618
- Mahmoud AM et al. Hyperinsulinemia augments endothelin-1 protein expression and impairs vasodilation of human skeletal muscle arterioles. Physiol Rep 4:N/A (2016). WB . PubMed: 27796268
- Tagashira H et al. UVB Stimulates the Expression of Endothelin B Receptor in Human Melanocytes via a Sequential Activation of the p38/MSK1/CREB/MITF Pathway Which Can Be Interrupted by a French Maritime Pine Bark Extract through a Direct Inactivation of MSK1. PLoS One 10:e0128678 (2015). Human . PubMed: 26030901