Recombinant
RabMAb

Recombinant Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102)

Submit a review Q&A (3)References (3)

Overview

  • Product name

    Anti-Endothelin B Receptor/ET-B antibody [EPR7013]
    See all Endothelin B Receptor/ET-B primary antibodies

  • Description

    Rabbit monoclonal [EPR7013] to Endothelin B Receptor/ET-B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Endothelin B Receptor/ET-B aa 50-150. The exact sequence is proprietary.
    Database link: P24530
    (Peptide available as ab170241)

  • Positive control

    • Raji, HeLa, JAR, and 293T cell lysates; HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 2.21 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7013

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

    • Tissue specificity

      Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.

    • Involvement in disease

      Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
      Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
      Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.

    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.

    • Post-translational
      modifications

      Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ABCDS antibody
        • Ednra antibody
        • EDNRB antibody
        • EDNRB_HUMAN antibody
        • Endothelin B receptor antibody
        • Endothelin B receptor precursor antibody
        • Endothelin receptor Non selective type antibody
        • Endothelin receptor non-selective type antibody
        • Endothelin receptor type B antibody
        • ET B antibody
        • ET-B antibody
        • ET-BR antibody
        • ETB antibody
        • ETBR antibody
        • ETRB antibody
        • Hirschsprung disease 2 antibody
        • HSCR antibody
        • HSCR2 antibody
        • OTTHUMP00000018534 antibody
        • OTTHUMP00000178736 antibody
        • WS4A antibody
        see all

      Images

      • Western blot - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102)
        Western blot - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102)
        All lanes : Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102) at 1/10000 dilution (purified)

        Lane 1 : HeLa cell lysate
        Lane 2 : HEK293 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

        Predicted band size: 50 kDa
        Observed band size: 50 kDa



        Blocking buffer: 5% NFDM/TBST
        Dilution buffer: 5% NFDM/TBST

      • Immunoprecipitation - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102)
        Immunoprecipitation - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102)

        ab129102 (purified) at 1/40 immunoprecipitating Endothelin B receptor//ET-B in 10 μg 293T (Lanes 1 and 2, observed at 50 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

      • Western blot - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102)
        Western blot - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102)
        All lanes : Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102) at 1/1000 dilution (unpurified)

        Lane 1 : Raji cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : JAR cell lysate
        Lane 4 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Predicted band size: 50 kDa

      • Other - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102)
        Other - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      This product has been referenced in:

      • Gibson DA  et al. Intracrine Androgens Enhance Decidualization and Modulate Expression of Human Endometrial Receptivity Genes. Sci Rep 6:19970 (2016). Read more (PubMed: 26817618) »
      • Mahmoud AM  et al. Hyperinsulinemia augments endothelin-1 protein expression and impairs vasodilation of human skeletal muscle arterioles. Physiol Rep 4:N/A (2016). WB . Read more (PubMed: 27796268) »
      See all 3 Publications for this product

      Publishing research using ab129102? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Question

      Inquiry: I would like to check expression of ETBR on our MSC cells using FACS. Do I need to permeabilise cells or could be seen on the surface by FACS? If need to permeabilise, what is the recommended procedure to do it? If you more information - pictures, concentration, method - that would be helpful.

      Read More
      Answer

      I am happy to confirm that you do not need to permeabilise your cells, since a peptide fom the extracellular region of the Endothelin B Receptor protein was used as immunogen.

      As additional information you can find attached data from Flow cytometric analysis using ab129102. Further, I detail a FC protocol, in case you find it useful:



      FC protocol (extracellular)



      1. Solutions and Reagents

      1.1. 1× PBS

      1.2. Blocking buffer: 3% FBS in 1X PBS



      2 Protocol

      2.1. Collect 1x106 cells/sample.

      2.2. Wash cells twice with blocking buffer.

      2.3. Add primary antibody at appropriate dilution and incubate for 1h at room temperature.

      2.4. Wash twice with blocking buffer and incubate with fluorescent-conjugated secondary antibody for 30 minutes at room temperature in dark.

      2.5. Wash twice with blocking buffer.

      2.6. Re-suspend cells in 1× PBS and transfer to FACS tubes. Analyze on flow cytometry.





      I

      Read More

      Question

      We performed western blot experiments and we would like to inform you that the peptide blocked successfully all three bands, therefore we assume that they are different isoforms of the receptor.

      Read More
      Answer

      Thank you very much for this information. We are very pleased to hear that you managed to determine the specificity of the anti-Endothelin Receptor B using the blocking peptide.

      Read More

      Question

      Dear All,
      we have a customer interested in your ab129102. In particular she would use in in flow cytometry experiments and she would like to get some infomation?
      - Which is the epitope recognise by the antibody (is an into or an extracellular epitope)?
      - Which conjugated Ab do you suggest as secondary Ab to use with ab129102 in flow cytometry?
      - Which isotype should I use as negative control?
      -Can you please provide the codes?

      Thanks in advance and kind regards

      Read More
      Answer

      Thank you for your inquiry.

      We currently don't determine the specific isotype for our rabbit monoclonal antibodies, and that they are simply known as IgG. We used rabbit IgG as negative control and we are offering the rabbit IgG, which can be used as a negative control for Flow Cytometry and IHC:

      https://www.abcam.com/index.html?c=718

      ab37406. ab37407, ab37415

      The exact immunogen sequence used to generate this antibody is commercially sensitive information.



      If you need any further assistance in the future, please do not hesitate to contact me.

      Read More

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