• Product name
    Anti-Endothelium antibody [PAL-E]
    See all Endothelium primary antibodies
  • Description
    Mouse monoclonal [PAL-E] to Endothelium
  • Host species
  • Specificity
    Reactivity with: Lymphatic endothelium :not detectable. Sinus histiocytes :not detectable. Megakaryocytes :not detectable.
  • Tested applications
    Suitable for: IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Sheep, Rabbit, Goat, Dog, Human, Pig
    Does not react with: Mouse, Rat, Chicken
  • Immunogen

    Human endothelium

  • General notes

    The antibody only stains human and various animal (goat, rabbit, pig, dog) blood vessels with the exception of arteries.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.07% Sodium azide
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    The antibody only stains human and various animal (goat, rabbit, pig, dog) blood vessels with the exception of arteries.
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab8086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100. Fix with acetone.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      The endothelium is located at the interface between the blood and the vessel wall. The cells are in close contact and form a slick layer that prevents blood cell interaction with the vessel wall as blood moves through the vessel lumen. The endothelium consists of simple squamous epithelium that lines the lumen of all blood vessels. It plays a critical role in the mechanics of blood flow, the regulation of coagulation, leukocyte adhesion, and vascular smooth muscle cell growth, and also serves as a barrier to the transvascular diffusion of liquids and solutes. For years the endothelium was thought of as an inert single layer of cells that passively allow the passage of water and other small molecules across the vessel wall. However, this dynamic tissue performs many other active functions, such as the secretion and modification of vasoactive substances and the contraction and relaxation of vascular smooth muscle.


    This product has been referenced in:
    • Elkadri A  et al. Mutations in Plasmalemma Vesicle Associated Protein Result in Sieving Protein-Losing Enteropathy Characterized by Hypoproteinemia, Hypoalbuminemia, and Hypertriglyceridemia. Cell Mol Gastroenterol Hepatol 1:381-394.e7 (2015). Read more (PubMed: 26207260) »
    • Moriyama T  et al. The significance of caveolae in the glomeruli in glomerular disease. J Clin Pathol : (2011). Read more (PubMed: 21450752) »

    See all 6 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (endothelial (HUVECs))
    endothelial (HUVECs)
    Yes - triton-x100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

    Dr. S Della Bella

    Verified customer

    Submitted Nov 26 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunohistochemistry (Frozen sections)
    Sheep Tissue sections (Brain)
    Yes - triton-x
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 15°C

    Abcam user community

    Verified customer

    Submitted Aug 24 2012

    I am very pleased to hear you would like to take part in the testing discount scheme. I have generated the discount code as follows:
    Discount code: xxxxxxxxx Expiration date: 2nd December 2012
    Please quote this code in the "Additional comment...

    Read More

    Thank you for contacting us and your interest in our products.
    As you have seen from the datasheet of the anti-Endothelium antibody [PAL-E] (ab8086), the antibody has not as yet been used in detecting the ovine protein or IHC of paraffin embedded ...

    Read More

    DISCOUNT CODE: 0000000

    Expiration date: 03. November 2012
    I am very pleased to hear your customer would like to accept our offer and test ab8086 in ICC/IF.
    This code will give your customer 1 free primary antibody before the expirat...

    Read More

    Thank you and your customer very much for your interest in ab8086.
    To our knowledge, ab8086 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if your customer buys ab8086 now, tests it in ICC/IF and submits fee...

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    Thank you for your email.
    I can confirm that you are correct and ab8086 is the same clone as ab128058 and therefore the best alternative for your customer.
    Please let me know what application and species your customer is using if you have any...

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    Thank you for your enquiry. All the information we having regarding this antibody is located on the online datasheet. I am sorry but we do not have a protocol for tagging ICG with PAL-E. If you have any more questions, please contact us again.

    Dear Kavita datasheets. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you dec...

    Read More

    We do not have any data on the antibodies use in formalin-fixed paraffin embedded tissue and believe that it has not yet been tested in this application. If we can find further information we will contact you with this.

    1-10 of 11 Abreviews or Q&A


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