Overview

  • Product name
    Anti-Endothelium antibody [PAL-E]
    See all Endothelium primary antibodies
  • Description
    Mouse monoclonal [PAL-E] to Endothelium
  • Host species
    Mouse
  • Specificity
    Reactivity with: Lymphatic endothelium :not detectable. Sinus histiocytes :not detectable. Megakaryocytes :not detectable.
  • Tested applications
    Suitable for: IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Sheep, Rabbit, Goat, Dog, Human, Pig
    Does not react with: Mouse, Rat, Chicken
  • Immunogen

    Human endothelium

  • General notes


    The antibody only stains human and various animal (goat, rabbit, pig, dog) blood vessels with the exception of arteries.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.07% Sodium azide
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    The antibody only stains human and various animal (goat, rabbit, pig, dog) blood vessels with the exception of arteries.
  • Clonality
    Monoclonal
  • Clone number
    PAL-E
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100. Fix with acetone.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      The endothelium is located at the interface between the blood and the vessel wall. The cells are in close contact and form a slick layer that prevents blood cell interaction with the vessel wall as blood moves through the vessel lumen. The endothelium consists of simple squamous epithelium that lines the lumen of all blood vessels. It plays a critical role in the mechanics of blood flow, the regulation of coagulation, leukocyte adhesion, and vascular smooth muscle cell growth, and also serves as a barrier to the transvascular diffusion of liquids and solutes. For years the endothelium was thought of as an inert single layer of cells that passively allow the passage of water and other small molecules across the vessel wall. However, this dynamic tissue performs many other active functions, such as the secretion and modification of vasoactive substances and the contraction and relaxation of vascular smooth muscle.

    References

    This product has been referenced in:
    • Elkadri A  et al. Mutations in Plasmalemma Vesicle Associated Protein Result in Sieving Protein-Losing Enteropathy Characterized by Hypoproteinemia, Hypoalbuminemia, and Hypertriglyceridemia. Cell Mol Gastroenterol Hepatol 1:381-394.e7 (2015). Read more (PubMed: 26207260) »
    • Moriyama T  et al. The significance of caveolae in the glomeruli in glomerular disease. J Clin Pathol : (2011). Read more (PubMed: 21450752) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-10 of 11 Abreviews or Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (endothelial (HUVECs))
    Specification
    endothelial (HUVECs)
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton-x100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

    Dr. S Della Bella

    Verified customer

    Submitted Nov 26 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Sheep Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Acetone
    Permeabilization
    Yes - triton-x
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 15°C

    Abcam user community

    Verified customer

    Submitted Aug 24 2012

    Answer

    I am very pleased to hear you would like to take part in the testing discount scheme. I have generated the discount code as follows:
    Discount code: xxxxxxxxx Expiration date: 2nd December 2012
    Please quote this code in the "Additional comments" section when submitting your Abreview for ab8086 with your ovine samples in IHC of paraffin embedded sections. Once the Abreview has been submitted the code will become activated and you can quote the code when placing the order for your free product. This code expires on the 2nd of December 2012.
    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.
    Until then, I wish you all the best with your research.

    Read More

    Answer

    Thank you for contacting us and your interest in our products.
    As you have seen from the datasheet of the anti-Endothelium antibody [PAL-E] (ab8086), the antibody has not as yet been used in detecting the ovine protein or IHC of paraffin embedded sections as you are hoping to do. This does not mean that it would not work, simply that we have not had any reports supporting it yet.
    Unfortunately we are not able to provide sample sizes of any of our products. This is due to the logistics that would be involved in this, with such a large catalogue this simply isn't feasible. However, we do offer a testing discount scheme. This offer means that if you test the antibody with ovine paraffin embedded tissue and share the results with us through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price off any product in our catalogue). More information on this offer can be found here:
    www.abcam.com/collaborationdiscount
    The only constraint to this offer is that the antibody to be tested must be purchased, tested, the Abreview submitted and the free primary antibody claimed within a 4 month period.
    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab8086.
    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Answer

    DISCOUNT CODE: 0000000

    Expiration date: 03. November 2012
    I am very pleased to hear your customer would like to accept our offer and test ab8086 in ICC/IF.
    This code will give your customer 1 free primary antibody before the expiration date.
    To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.
    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
    1) Call to place your order and mention the code to our customer service department;
    2) Include the code in your fax order;
    3) Place your order on the web and enter the promotional code.
    Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you and your customer very much for your interest in ab8086.
    To our knowledge, ab8086 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if your customer buys ab8086 now, tests it in ICC/IF and submits feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your customer's feedback. The discount would be to the value of ab8086 and can be used for the next order of any product.
    If your customer is interested in this offer, please follow these steps:
    1. Reply to this e-mail to let me know that your customer ( please include their e-mail address) would like to proceed and test ab8086 in ICC/IF. I will then send a discount code. This code must be issued before purchasing ab8086 so please wait for my reply before ordering.
    2. Purchase ab8086 either by phone, fax, or online (www.abcam.com).
    3. Test it in ICC/IF.
    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
    5. After the review is submitted to us, the discount code becomes active. Simply place your customer's new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab8086 turns out to be unsuitable for ICC/IF, your customer will still receive the discount on their next purchase after your Abreview has been submitted.
    Please let me know if you or your customer have any questions about this offer and I would be happy to help you further.
    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you for your email.
    I can confirm that you are correct and ab8086 is the same clone as ab128058 and therefore the best alternative for your customer.
    Please let me know what application and species your customer is using if you have any more questions in this matter.

    Read More

    Answer

    Thank you for your enquiry. All the information we having regarding this antibody is located on the online datasheet. I am sorry but we do not have a protocol for tagging ICG with PAL-E. If you have any more questions, please contact us again.

    Read More

    Answer

    Dear Kavita datasheets. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

    Read More

    Answer

    We do not have any data on the antibodies use in formalin-fixed paraffin embedded tissue and believe that it has not yet been tested in this application. If we can find further information we will contact you with this.

    Read More

    1-10 of 11 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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