Overview

  • Product name

    Endotoxin Removal Kit (Rapid)
  • Product overview

    Endotoxin Removal Kit (Rapid)(ab239707) can quickly and effectively eliminate endotoxins to < 0.05 EU/ml in solutions containing proteins or pharmacologically important components via the immobilized polymyxin B, which is known for capturing endotoxin and preventing toxic effects.


    The kit quickly and effectively eliminates endotoxins to < 0.05 EU/ml and is ideal for processing small-scale solution sample (0.1-0.5 mL).

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 5 kit
    Endotoxin Removal Equilibration Buffer 1 x 10ml
    Endotoxin-free Collection Tube 10 units
    Rapid Endotoxin Removal Regeneration Buffer 2 x 10ml
    Rapid Endotoxin Removal Spin Column 5 units
    Rapid Removal Wash Buffer 1 x 10ml
  • Research areas

  • Relevance

    Endotoxins are part of the outer membrane of the cell wall of gram negative bacteria. Endotoxins are invariably associated with gram negative bacteria whether the organisms are pathogens or not. Although the term "endotoxin" is occasionally used to refer to any cell associated bacterial toxin, it is properly reserved to refer to the lipopolysaccharide complex associated with the outer membrane of gram-negative bacteria such as E. coli, Salmonella, Shigella, Pseudomonas, Neisseria, Haemophilus, and other leading pathogens. The biological activity of endotoxins are associated with lipopolysaccharide (LPS). Toxicity is associated with the lipid component (Lipid A) and immunogenicity is associated with the polysaccharide components. The cell wall antigens (O antigens) of gram negative bacteria are components of LPS. LPS elicits a variety of inflammatory responses in an animal. Because it activates complement by the alternative (properdin) pathway, it is often part of the pathology of gram negative bacterial infections.
  • Cellular localization

    Cell Membrane
  • Alternative names

    • E coli LPS

Images

  • Endotoxin capacities and endotoxin efficiencies were determined by challenging 0.1 mL resin with 1 x 108 EU/mL LPS in 200 µL BSA (10 mg/mL). By reloading samples to the repeatedly regenerated column, the endotoxin spike is reduced to <0.05 EU/mL in the BSA solution. The highest endotoxin capacity is 9.99 x 108 EU/mL from the first cycle of detox. The average efficiency of 5 cycles of detox is 94.5% and the average protein recovery from 5 cycles of detox is 89.9%.

Protocols

References

ab239707 has not yet been referenced specifically in any publications.

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