Overview

  • Product name

    Anti-Engrailed and Invected antibody [4D9]
  • Description

    Mouse monoclonal [4D9] to Engrailed and Invected
  • Host species

    Mouse
  • Specificity

    This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons.
  • Tested applications

    Suitable for: IHC - Wholemountmore details
  • Species reactivity

    Reacts with: Chicken, Xenopus laevis, Drosophila melanogaster, Zebrafish, Crayfish
    Does not react with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Drosophila melanogaster Engrailed and Invected (C terminal).

  • Epitope

    Epitope has been localized to residues 38-58 of the homeodomain.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    4D9
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount 1/200.

Fix with 4% paraformaldehyde in PBS for 1 hour.

 

Target

  • Relevance

    In Drosophila, the Engrailed gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Expression studies have suggested that Engrailed may also function early in organizing the preblastoderm and later in neurogenesis. Engrailed encodes a homeodomain-containing protein that can function as a transcription factor. Invected is homologous to Engrailed and the gene lies adjacent to that for Engrailed. The function of Invected is unclear.
  • Database links

    • Alternative names

      • En antibody
      • Eng antibody
      • Engrailed antibody
      • Engrailed/Invected antibody
      • Homeobox protein invected antibody
      • Inv antibody
      • Invected antibody
      see all

    Images

    • ab12454 used in immunohistochemistry at 1/200 on Drosophila embryos. The protocol used is available here.

      Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with DAB.

    • ab12454 used in immunofluorescence at 1/200 on Drosophila embryos. The protocol used is available here.

      Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining.

    • ab12454 used in immunohistochemistry (Wholemount) at 1/200 dilution on Drosophila embryos. The protocol used is available here.

      Briefly, embryos were bleached (5% Bleach) to dechorionate them, followed by fixation in PBS, 3.7% formaldehyde and n-Heptane, dehydration with Methanol, rehydration with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with DAB.

    References

    This product has been referenced in:

    • Baratte S  et al. Engrailed in cephalopods: a key gene related to the emergence of morphological novelties. Dev Genes Evol 217:353-62 (2007). Read more (PubMed: 17394016) »
    • Harzsch S Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida). Dev Biol 258:44-56 (2003). Read more (PubMed: 12781681) »
    See all 11 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample
    Fruit fly (Drosophila melanogaster) Cell (Drosophila Wing Imaginal disc)
    Specification
    Drosophila Wing Imaginal disc
    Permeabilization
    Yes - 0.5% Triton X100
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 16 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up