This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200. Fix with 4% paraformaldehyde in PBS for 1 hour.
In Drosophila, the Engrailed gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Expression studies have suggested that Engrailed may also function early in organizing the preblastoderm and later in neurogenesis. Engrailed encodes a homeodomain-containing protein that can function as a transcription factor.
Invected is homologous to Engrailed and the gene lies adjacent to that for Engrailed. The function of Invected is unclear.
ab12454 used in immunohistochemistry at 1/200 on Drosophila embryos. The protocol used is available here.
Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.Visualisation was with DAB.
Baratte S et al. Engrailed in cephalopods: a key gene related to the emergence of morphological novelties. Dev Genes Evol217:353-62 (2007).
Read more (PubMed: 17394016) »
Harzsch S Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida). Dev Biol258:44-56 (2003).
Read more (PubMed: 12781681) »