Overview

  • Product name

    Enhanced Apoptotic DNA Ladder Detection Kit
    See all DNA fragmentation kits
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Assay type

    Direct
  • Assay time

    1h 30m
  • Product overview

    Enhanced Apoptotic DNA Ladder Detection Kit (ab66093) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells. It can isolate small fragmented DNA from cells in only 90 minutes, whereas other similar products in the market require 1-2 days. No DNA extraction or additional columns required. DNA fragments can be easily visualized by agarose gel electrophoresis stained with a highly sensitive dye.

  • Notes

    For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.

Properties

Protocols

References

ab66093 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Warm to 37C while pipetting up and down and this should help. If the pellet was over-dried at the step prior, then this can happen. If the DNA still does not solubilize, the suspension can be heated to 65C with pipetting. Increasing the 50C incubation with Enzyme B in step 5 to overnight rather than 30 minutes should help prevent this from happening as well.

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