Anti-Enhanced Blue Fluorescent Protein antibody [3A6] (ab32791)

Reviews (1)Q&A (4)References (2)

Overview

  • Product name

    Anti-Enhanced Blue Fluorescent Protein antibody [3A6]

  • Description

    Mouse monoclonal [3A6] to Enhanced Blue Fluorescent Protein

  • Host species

    Mouse

  • Specificity

    Cross reacts with GFP and YFP. Additional tests for cross reactivity have not been performed.

  • Tested applications

    Suitable for: ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.

Properties

  • Form

    Lyophilised:Dissolve the antibody in a Tris HCl pH 8.0 buffer, containing 0.05% sodium azide (dissolving 100 µg of antibody in 200 µl of buffer will result in a concentration of 0.5 mg/ml).

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.

  • Storage buffer

    pH: 8.00
    Preservative: 0.05% Sodium azide
    Constituent: 1.58% Tris HCl
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    3A6

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: 1/1000F - 1/2000F.
    For the value of the multiplication factor F, see label on vial.
    Unless the stability in the actual test system has been established, it is
    recommended to dilute the product immediately before use.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.

    References

    This product has been referenced in:

    • Ling SC  et al. Overriding FUS autoregulation in mice triggers gain-of-toxic dysfunctions in RNA metabolism and autophagy-lysosome axis. Elife 8:N/A (2019). Read more (PubMed: 30747709) »
    • Giannotti G  et al. Divergent Prelimbic Cortical Pathways Interact with BDNF to Regulate Cocaine-seeking. J Neurosci 38:8956-8966 (2018). Read more (PubMed: 30185459) »
    See all 2 Publications for this product

    Publishing research using ab32791? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry free floating abreview for Anti-Enhanced Blue Fluorescent Protein antibody [3A6]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 19 2016

    Question

    Does it work for western blot?

    Read More
    Answer

    Thank you for your reply.

    To our knowledge, ab32791 has not been tested in WB. Therefore, I can offer a discount off a future purchase if you buy ab32791now, test it inWB and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab32791 in WB. I will then send a discount code.

    2. Purchase ab32791 either by phone, fax, or online (www.abcam.com).

    3. Test it in WB.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab32791 turns out to be unsuitable for WB, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Question

    I want to order Anti-mouse GFP antibody (ab1218) for quantification of GFP and BFP protein expression. Does it react with BFP? Please let me know as soon as possible. Thanks.

    Read More
    Answer

    Thank you for contacting us.

    We have not tested ab1218 for reactivity with BFP. It is possible that this antibody may react with BFP, but as we have not mapped the epitope for this antibody, I do not have any further information about whether this antibody would be likely to react or not. We do have an antibody (ab32791) to EBFP that would be suitable for measuring both BFP and GFP. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Question

    Dear Sir or Madame,
    I am looking for Antibodies against Fluorescent Proteins to be used in Immunohistochemistry. I want to perform tripple-stainings against GFP, dsRed and an additional fluorescent Protein.
    Which additional fluorescent Protein and which of your ABs would you recommend?
    Ideally it can be combined with your Living Color dsred Polyclonal (raised in rabbit, Cat. 632496) and an anti-GFP (raised in chicken) which we are currently using to stain for our constructs containing the dsRed- and GFP-protein.
    Best regards,

    Read More
    Answer

    Thank you for contacting us.

    We are happy to help customers find the most suitable products for their research purposes; however there are many fluorescent proteins and we are not specialized in this particular field. We donot want to recommend a product that may be unsuitable or not the best choice. I would thereforeencourageyou to consult the latest literature available through PubMed and other resources in order to find the most adapted fluorescent protein to complement your immunohistochemistry.

    Improved blue fluorescent proteins are for example described here:
    http://www.ncbi.nlm.nih.gov/pubmed/17444659

    Additional information source may be ˜
    http://www.scholarpedia.org/article/Fluorescent_proteins
    http://www.nature.com/nature/journal/v450/n7166/abs/nature06293.html
    http://www.conncoll.edu/ccacad/zimmer/GFP-ww/GFP-1.htm


    As antibodies, in addition to all the anti GFP/EGFP/YFP/RFP antibodies,we do have an antibody against the blue fluorescent protein ab32791, which is a mouse monoclonal antibody or the anti aequorin antibody, which is a rabbit polyclonal, ab9096.
    https://www.abcam.com/index.html?datasheet=32791 (or use the following: https://www.abcam.com/index.html?datasheet=32791).
    https://www.abcam.com/index.html?datasheet=9096 (or use the following: https://www.abcam.com/index.html?datasheet=9096).

    I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again if you would need some more specific information on any of our products.

    I wish you good success withyour research!

    Read More

    Question

    I am hoping to buy ab32791 (anti-enhanced blue fluoroscent protein antibody). I would like to know if I can get that already reconstituted instead of the lyophilised form

    Read More
    Answer

    Thank you for contacting us.

    We typically sell this antibody in lyophilized form to increase it's storage stability and shelf-life. We may be able to offer it in liquid form, but custom formulations are normally only available for bulk orders. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

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