Anti-Enhanced Blue Fluorescent Protein antibody [3A6] (ab32791)
Key features and details
- Mouse monoclonal [3A6] to Enhanced Blue Fluorescent Protein
- Suitable for: ELISA
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-Enhanced Blue Fluorescent Protein antibody [3A6]
See all Blue Fluorescent Protein primary antibodies -
Description
Mouse monoclonal [3A6] to Enhanced Blue Fluorescent Protein -
Host species
Mouse -
Specificity
Cross reacts with GFP and YFP. Additional tests for cross reactivity have not been performed. -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Dissolve the antibody in a Tris HCl pH 8.0 buffer, containing 0.05% sodium azide (dissolving 100 µg of antibody in 200 µl of buffer will result in a concentration of 0.5 mg/ml). -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 8.00
Preservative: 0.05% Sodium azide
Constituent: 1.58% Tris HCl -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3A6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/1000 - 1/2000.
1/1000F - 1/2000F. For the value of the multiplication factor F, see label on vial. Unless the stability in the actual test system has been established, it is recommended to dilute the product immediately before use. |
Notes |
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ELISA
1/1000 - 1/2000. 1/1000F - 1/2000F. For the value of the multiplication factor F, see label on vial. Unless the stability in the actual test system has been established, it is recommended to dilute the product immediately before use. |
Target
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Relevance
Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems. -
Alternative names
- BFP antibody
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab32791 has been referenced in 2 publications.
- Ling SC et al. Overriding FUS autoregulation in mice triggers gain-of-toxic dysfunctions in RNA metabolism and autophagy-lysosome axis. Elife 8:N/A (2019). PubMed: 30747709
- Giannotti G et al. Divergent Prelimbic Cortical Pathways Interact with BDNF to Regulate Cocaine-seeking. J Neurosci 38:8956-8966 (2018). PubMed: 30185459