Key features and details
- Mouse monoclonal [3A6] to Enhanced Blue Fluorescent Protein
- Suitable for: ELISA
- Reacts with: Species independent
- Isotype: IgG1
Product nameAnti-Enhanced Blue Fluorescent Protein antibody [3A6]
DescriptionMouse monoclonal [3A6] to Enhanced Blue Fluorescent Protein
SpecificityCross reacts with GFP and YFP. Additional tests for cross reactivity have not been performed.
Tested applicationsSuitable for: ELISAmore details
Species reactivityReacts with: Species independent
Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
FormLyophilized:Dissolve the antibody in a Tris HCl pH 8.0 buffer, containing 0.05% sodium azide (dissolving 100 µg of antibody in 200 µl of buffer will result in a concentration of 0.5 mg/ml).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferpH: 8.00
Preservative: 0.05% Sodium azide
Constituent: 1.58% Tris HCl
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab32791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For the value of the multiplication factor F, see label on vial.
Unless the stability in the actual test system has been established, it is
recommended to dilute the product immediately before use.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceFluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab32791 has been referenced in 2 publications.
- Ling SC et al. Overriding FUS autoregulation in mice triggers gain-of-toxic dysfunctions in RNA metabolism and autophagy-lysosome axis. Elife 8:N/A (2019). PubMed: 30747709
- Giannotti G et al. Divergent Prelimbic Cortical Pathways Interact with BDNF to Regulate Cocaine-seeking. J Neurosci 38:8956-8966 (2018). PubMed: 30185459