Anti-Enhanced Blue Fluorescent Protein antibody [3A6] (ab32791)

Abreviews (1)Q&A (4)

Overview

  • Product name
    Anti-Enhanced Blue Fluorescent Protein antibody [3A6]
  • Description
    Mouse monoclonal [3A6] to Enhanced Blue Fluorescent Protein
  • Host species
    Mouse
  • Specificity
    Cross reacts with GFP and YFP. Additional tests for cross reactivity have not been performed.
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.

Properties

  • Form
    Lyophilised:Dissolve the antibody in a Tris HCl pH 8.0 buffer, containing 0.05% sodium azide (dissolving 100 µg of antibody in 200 µl of buffer will result in a concentration of 0.5 mg/ml).
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer
    pH: 8.00
    Preservative: 0.05% Sodium azide
    Constituent: 1.58% Tris HCl
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    3A6
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: 1/1000F - 1/2000F.
    For the value of the multiplication factor F, see label on vial.
    Unless the stability in the actual test system has been established, it is
    recommended to dilute the product immediately before use.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance
      Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.

    References

    ab32791 has not yet been referenced specifically in any publications.

    Publishing research using ab32791? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    5 reviews or Q&A

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    Votes: Highest First

    Immunohistochemistry free floating abreview for Anti-Enhanced Blue Fluorescent Protein antibody [3A6]

     Excellent
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
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    Username

    Abcam user community

    Verified customer

    Submitted Dec 19 2016

    Does it work for western blot?

    Thank you for your reply.

    To our knowledge, ab32791 has not been tested in WB. Therefore, I can offer a discount off a future purchase if you buy ab32791now, test it inWB and submit feedback to us in the form of an Abreview. It doesn’t...

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    I want to order Anti-mouse GFP antibody (ab1218) for quantification of GFP and BFP protein expression. Does it react with BFP? Please let me know as soon as possible. Thanks.

    Thank you for contacting us.

    We have not tested ab1218 for reactivity with BFP. It is possible that this antibody may react with BFP, but as we have not mapped the epitope for this antibody, I do not have any further information about whether...

    Read More

    Dear Sir or Madame,
    I am looking for Antibodies against Fluorescent Proteins to be used in Immunohistochemistry. I want to perform tripple-stainings against GFP, dsRed and an additional fluorescent Protein.
    Which additional fluorescent Protei...

    Read More

    Thank you for contacting us.

    We are happy to help customers find the most suitable products for their research purposes; however there are many fluorescent proteins and we are not specialized in this particular field. We donot want to recomm...

    Read More

    I am hoping to buy ab32791 (anti-enhanced blue fluoroscent protein antibody). I would like to know if I can get that already reconstituted instead of the lyophilised form

    Thank you for contacting us.

    We typically sell this antibody in lyophilized form to increase it's storage stability and shelf-life. We may be able to offer it in liquid form, but custom formulations are normally only available for bulk orders...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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