Anti-Enkephalin/ENK antibody (ab77273)
Key features and details
- Goat polyclonal to Enkephalin/ENK
- Suitable for: WB, IHC-FoFr
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Enkephalin/ENK antibody
See all Enkephalin/ENK primary antibodies -
Description
Goat polyclonal to Enkephalin/ENK -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-FoFrmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human Adrenal Gland lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
1 hour primary incubation is recommended for this product. |
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IHC-FoFr |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa). 1 hour primary incubation is recommended for this product. |
IHC-FoFr
Use at an assay dependent concentration. |
Target
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Function
Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. -
Sequence similarities
Belongs to the opioid neuropeptide precursor family. -
Post-translational
modificationsThe N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5179 Human
- Omim: 131330 Human
- SwissProt: P01210 Human
- Unigene: 339831 Human
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Alternative names
- ENK antibody
- Enkephalin A antibody
- Enkephalin antibody
see all
Images
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Anti-Enkephalin/ENK antibody (ab77273) at 0.3 µg/ml + Human Adrenal Gland lysate in RIPA buffer at 35 µg
Predicted band size: 31 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab77273 has been referenced in 2 publications.
- Shi MM et al. Hippocampal µ-opioid receptors on GABAergic neurons mediate stress-induced impairment of memory retrieval. Mol Psychiatry 25:977-992 (2020). PubMed: 31142818
- Tang D et al. High expression of proenkephalin is associated with favorable outcomes in patients with gastrointestinal stromal tumors. Cancer Manag Res 11:6681-6690 (2019). PubMed: 31410059