Key features and details
- Goat polyclonal to Enkephalin/ENK
- Suitable for: WB, IHC-FoFr
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Enkephalin/ENK antibody
See all Enkephalin/ENK primary antibodies
DescriptionGoat polyclonal to Enkephalin/ENK
Tested applicationsSuitable for: WB, IHC-FoFrmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
- Human Adrenal Gland lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
1 hour primary incubation is recommended for this product.
|IHC-FoFr||Use a concentration of 0.3 - 1 µg/ml.|
FunctionMet- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
Sequence similaritiesBelongs to the opioid neuropeptide precursor family.
modificationsThe N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
- Information by UniProt
- ENK antibody
- Enkephalin A antibody
- Enkephalin antibody
Anti-Enkephalin/ENK antibody (ab77273) at 0.3 µg/ml + Human Adrenal Gland lysate in RIPA buffer at 35 µg
Predicted band size: 31 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
ab77273 has been referenced in 2 publications.
- Shi MM et al. Hippocampal µ-opioid receptors on GABAergic neurons mediate stress-induced impairment of memory retrieval. Mol Psychiatry 25:977-992 (2020). PubMed: 31142818
- Tang D et al. High expression of proenkephalin is associated with favorable outcomes in patients with gastrointestinal stromal tumors. Cancer Manag Res 11:6681-6690 (2019). PubMed: 31410059