Anti-Enkephalin/ENK antibody (ab85798)
Key features and details
- Rabbit polyclonal to Enkephalin/ENK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Enkephalin/ENK antibody
See all Enkephalin/ENK primary antibodies -
Description
Rabbit polyclonal to Enkephalin/ENK -
Host species
Rabbit -
Specificity
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Enkephalin/ENK aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab102738) -
Positive control
- This antibody gave a positive signal in Human testis tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. -
Sequence similarities
Belongs to the opioid neuropeptide precursor family. -
Post-translational
modificationsThe N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5179 Human
- Entrez Gene: 18619 Mouse
- Entrez Gene: 29237 Rat
- Omim: 131330 Human
- SwissProt: P01210 Human
- SwissProt: P22005 Mouse
- SwissProt: P04094 Rat
- Unigene: 339831 Human
see all -
Alternative names
- ENK antibody
- Enkephalin A antibody
- Enkephalin antibody
see all
Images
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Anti-Enkephalin/ENK antibody (ab85798) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThe band observed at 28 kDa could potentially be a cleaved form of Enkephalin due to the presence of a 24 amino acid signal peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab85798 has been referenced in 3 publications.
- Cai H et al. Inhibition of endoplasmic reticulum stress reverses synaptic plasticity deficits in striatum of DYT1 dystonia mice. Aging (Albany NY) 13:20319-20334 (2021). PubMed: 34398825
- Creus-Muncunill J et al. Huntington's disease brain-derived small RNAs recapitulate associated neuropathology in mice. Acta Neuropathol 141:565-584 (2021). PubMed: 33547932
- Bulc M et al. Changes in Immunoreactivity of Sensory Substances within the Enteric Nervous System of the Porcine Stomach during Experimentally Induced Diabetes. J Diabetes Res 2018:4735659 (2018). PubMed: 30140706