Overview

  • Product name
    Anti-Enkephalin/ENK antibody [NOC1]
    See all Enkephalin/ENK primary antibodies
  • Description
    Mouse monoclonal [NOC1] to Enkephalin/ENK
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, RIA, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Leu5-enkephalin conjugated to BSA

Properties

Applications

Our Abpromise guarantee covers the use of ab150346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
IHC-Fr Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
ICC 1/200.

Target

  • Function
    Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
  • Sequence similarities
    Belongs to the opioid neuropeptide precursor family.
  • Post-translational
    modifications
    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ENK antibody
    • Enkephalin A antibody
    • Enkephalin antibody
    • Met-enkephalin-Arg-Phe antibody
    • OGF antibody
    • Opioid growth factor antibody
    • PENK antibody
    • PENK_HUMAN antibody
    • Preproenkephalin antibody
    • Proenkephalin A antibody
    • Proenkephalin antibody
    see all

Images

  • Paraformaldehyde-fixed, paraffin-embedded mouse brain tissue stained for Enkephalin using ab150346 at 1/100 dilution in immunohistochemical analysis, followed by goat anti-mouse Alexa Fluor® 488.

    See Abreview

References

This product has been referenced in:
  • Cuello AC  et al. Characterization and immunocytochemical application of monoclonal antibodies against enkephalins. J Histochem Cytochem 32:947-57 (1984). Read more (PubMed: 6086744) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain(striatum))
Specification
Brain(striatum)
Username

Dr. Hyun Jin Kim

Verified customer

Submitted Apr 27 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (forebrain striatum)
Antigen retrieval step
Other
Permeabilization
Yes - 0.2% triton
Specification
forebrain striatum
Blocking step
various tested incl combinations of BSA serum, gelatin as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Heike Rebholz

Verified customer

Submitted Aug 18 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.5% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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