Key features and details
- Rabbit polyclonal to ENL
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ENL antibody
See all ENL primary antibodies
DescriptionRabbit polyclonal to ENL
Tested applicationsSuitable for: IP, WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ENL aa 325-375. The exact sequence is proprietary. NP_005925.2
Database link: Q03111
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human testicular seminoma tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245520 was affinity purified using an epitope specific to ENL immobilized on solid support.
Our Abpromise guarantee covers the use of ab245520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 3-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 62 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCapable of activating transcription from synthetic reporter genes in both lymphoid and myeloid cells.
Involvement in diseaseNote=A chromosomal aberration involving MLLT1 is associated with acute leukemias. Translocation t(11;19)(q23;p13.3) with MLL/HRX. The result is a rogue activator protein.
Sequence similaritiesContains 1 YEATS domain.
- Information by UniProt
- ENL antibody
- ENL_HUMAN antibody
- LTG19 antibody
Formalin-fixed, paraffin-embedded human testicular seminoma tissue stained for ENL using ab245520 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
All lanes : Anti-ENL antibody (ab245520) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
Exposure time: 30 seconds
ENL was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245520 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245520 at 0.4 µg/ml.
Lane 1: ab245520 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245520 has not yet been referenced specifically in any publications.