Overview

  • Product name

  • Description

    Rabbit polyclonal to ENL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ENL aa 325-375. The exact sequence is proprietary. NP_005925.2
    Database link: Q03111

  • Positive control

    • WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human testicular seminoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab245520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 3-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Capable of activating transcription from synthetic reporter genes in both lymphoid and myeloid cells.
  • Involvement in disease

    Note=A chromosomal aberration involving MLLT1 is associated with acute leukemias. Translocation t(11;19)(q23;p13.3) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similarities

    Contains 1 YEATS domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ENL antibody
    • ENL_HUMAN antibody
    • LTG19 antibody
    • MLLT1 antibody
    • Myeloid/lymphoid or mixed lineage leukemia trithorax (Drosophila) antibody
    • Protein ENL antibody
    • YEATS domain containing protein 1 antibody
    • YEATS domain-containing protein 1 antibody
    • YEATS1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human testicular seminoma tissue stained for ENL using ab245520 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

  • All lanes : Anti-ENL antibody (ab245520) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 62 kDa


    Exposure time: 30 seconds
  • ENL was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245520 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245520 at 0.4 µg/ml.

    Lane 1: ab245520 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab245520 has not yet been referenced specifically in any publications.

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