Recombinant Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18422] to ENO1 + ENO2 + ENO3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422]
See all ENO1 + ENO2 + ENO3 primary antibodies -
Description
Rabbit monoclonal [EPR18422] to ENO1 + ENO2 + ENO3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human ENO1 full length recombinant protein, Human ENO2 full length recombinant protein, Human ENO3 full length recombinant protein, Human fetal liver lysate, Human fetal heart lysate, Human fetal kidney lysate, Human fetal spleen lysate, Mouse brain lysate, Mouse heart lysate, Mouse kidney lysate, Mouse spleen lysate, Rat brain lysate, Rat heart lysate, Rat spleen lysate, HeLa, Jurkat MCF7 , A431, C6, Raw264.7 and NIH/3T3 whole cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18422 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
Target
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Relevance
Enolase 1 is a multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. Enolase 2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. Enolase 3 appears to have a function in striated muscle development and regeneration. -
Cellular localization
ENO1: Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M-band. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M-band. ENO2: Cytoplasm. Cell membrane. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form ENO3: Cytoplasm. Note: Localized to the Z line. Some colocalization with CKM at M-band. -
Database links
- Entrez Gene: 2023 Human
- Entrez Gene: 2026 Human
- Entrez Gene: 2027 Human
- Entrez Gene: 13806 Mouse
- Entrez Gene: 13807 Mouse
- Entrez Gene: 13808 Mouse
- Entrez Gene: 24333 Rat
- Entrez Gene: 24334 Rat
see all -
Alternative names
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha-enolase antibody
- Beta-enolase antibody
see all
Images
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All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/5000 dilution
Lane 1 : Human ENO1 full length recombinant protein
Lane 2 : Human ENO2 full length recombinant protein
Lane 3 : Human ENO3 full length recombinant protein
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 5 secondsBlocking and diluting buffer 5% NFDM /TBST.
Human ENO1 full length recombinant protein (Cat#:ab89248) containing aa1-434.
Human ENO2 full length recombinant protein containing aa1-434 with a His-Tag®.
Human ENO3 full length recombinant protein (Cat#:ab113127) containing aa1-434 with a His-Tag®.Human ENO2 full length recombinant protein was made in-house.
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All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/5000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lane 4 : Human fetal spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 5 secondsBlocking and diluting buffer 5% NFDM /TBST.
Exposure time - Lane 1,2 and 3:5 seconds;
Lane 4: 30 seconds -
All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 3 secondsBlocking and diluting buffer was 5% NFDM /TBST.
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Lanes 1-7 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/5000 dilution
Lanes 8-10 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR18422] (ab189892) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat spleen lysate
Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 9 : Raw264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 10 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 1 secondBlocking and diluting buffer was 5% NFDM /TBST.
Exposure time - Lane 1-4 and 8-10: 1 second;
Lane 5-7: 3 seconds;
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab189892 has not yet been referenced specifically in any publications.