Overview

  • Product name

    Anti-ENO1 antibody [253] - N-terminal
    See all ENO1 primary antibodies
  • Description

    Mouse monoclonal [253] to ENO1 - N-terminal
  • Host species

    Mouse
  • Specificity

    ab190365 was tested for binding to expressed bovine ENO1, ENO2 and ENO3 and shown to be specific for only ENO1.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Cow
    Predicted to work with: Mouse, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Cow ENO1 (N terminal). Immunogen corresponds to 12 amino acids synthesized on a 8-amine lysine core using the multiple antigen presentation method.
    Database link: Q9XSJ4

  • Positive control

    • HeLa cell lysate and rat 3T3 cells.

Applications

Our Abpromise guarantee covers the use of ab190365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF 1/2000 - 1/5000.

Target

  • Function

    Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities

    Belongs to the enolase family.
  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2 phospho D glycerate hydro lyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Alpha enolase antibody
    • Alpha enolase like 1 antibody
    • Alpha-enolase antibody
    • C myc promoter binding protein antibody
    • C-myc promoter-binding protein antibody
    • EC 4.2.1.11 antibody
    • eno1 antibody
    • ENO1L1 antibody
    • ENOA_HUMAN antibody
    • Enolase 1 (alpha) antibody
    • Enolase 1 (alpha) like 1 antibody
    • Enolase 1 antibody
    • Enolase alpha antibody
    • MBP 1 antibody
    • MBP-1 antibody
    • MBP1 antibody
    • MBPB1 antibody
    • MPB 1 antibody
    • MPB-1 antibody
    • MPB1 antibody
    • MYC promoter binding protein 1 antibody
    • NNE antibody
    • Non neural enolase antibody
    • Non-neural enolase antibody
    • Phosphopyruvate hydratase antibody
    • Plasminogen binding protein antibody
    • Plasminogen-binding protein antibody
    • PPH antibody
    • Tau crystallin antibody
    see all

Images

  • Immunofluorescence analysis of rat 3T3 cells, labeling ENO1 using ab190365 at a 1/2000 dilution (green). Counterstained with an antibody against Vimentin (red) and DNA (blue).

  • Anti-ENO1 antibody [253] - N-terminal (ab190365) at 1/5000 dilution + HeLa cell lysate

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

References

This product has been referenced in:

  • Ye Y  et al. Anti a-enolase antibody is a novel autoimmune biomarker for unexplained recurrent miscarriages. EBioMedicine 41:610-622 (2019). Read more (PubMed: 30827932) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Na-Citrate Buffer, pH6.0
Permeabilization
No
Specification
placenta
Blocking step
ZytoChem Plus HRP Polymer System Reagent 1 as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Apr 16 2018

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