Anti-ENO1 antibody [2G2AG11BF8] (ab112994)

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Overview

  • Product name

    Anti-ENO1 antibody [2G2AG11BF8]
    See all ENO1 primary antibodies

  • Description

    Mouse monoclonal [2G2AG11BF8] to ENO1

  • Host species

    Mouse

  • Tested applications

    Suitable for: IP, In-Cell ELISA, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Non human primates

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • HeLa and HL60 cells; HeLa, Human Liver and Heart homogenates.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab112994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

  • Sequence similarities

    Belongs to the enolase family.

  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

  • Post-translational
    modifications

    ISGylated.

  • Cellular localization

    Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt

  • Database links

    • Alternative names

      • 2 phospho D glycerate hydro lyase antibody
      • 2-phospho-D-glycerate hydro-lyase antibody
      • Alpha enolase antibody
      • Alpha enolase like 1 antibody
      • Alpha-enolase antibody
      • C myc promoter binding protein antibody
      • C-myc promoter-binding protein antibody
      • EC 4.2.1.11 antibody
      • eno1 antibody
      • ENO1L1 antibody
      • ENOA_HUMAN antibody
      • Enolase 1 (alpha) antibody
      • Enolase 1 (alpha) like 1 antibody
      • Enolase 1 antibody
      • Enolase alpha antibody
      • MBP 1 antibody
      • MBP-1 antibody
      • MBP1 antibody
      • MBPB1 antibody
      • MPB 1 antibody
      • MPB-1 antibody
      • MPB1 antibody
      • MYC promoter binding protein 1 antibody
      • NNE antibody
      • Non neural enolase antibody
      • Non-neural enolase antibody
      • Phosphopyruvate hydratase antibody
      • Plasminogen binding protein antibody
      • Plasminogen-binding protein antibody
      • PPH antibody
      • Tau crystallin antibody
      see all

    Images

    • Immunoprecipitation - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)
      Immunoprecipitation - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)
      Immunprecipitation of ENO1 by ab112994.
      Staining of ENO1 (47 kDa) from HeLa cell lysate (1), Human liver (2) and Heart (3) homogenates. The identity of the protein was confirmed by mass spectrometry. This gel was stained with colloidal Coomassie blue G.
    • Flow Cytometry - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)
      Flow Cytometry - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)
      ab112994 at 1ug/ml staining ENO1 in MeOH-fixed HL60 cells by Flow Cytometry(blue). Isotype control antibody (red).
    • Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)
      Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)
      ab112994 at 5ug/ml staining ENO1 in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly to the cytoplasm.

    References

    This product has been referenced in:

    • Chen X  et al. Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells. Oncotarget 8:30305-30316 (2017). Read more (PubMed: 28415822) »
    See 1 Publication for this product

    Publishing research using ab112994? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Please confirm details of how this antibody was purified:
    ab112994 Anti-ENO1 antibody [2G2AG11BF8]

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    Answer

    Thank you for your recent telephone enquiry. I am sorry it has taken some time to obtain this information, I appreciate your patience.

    I can confirm that ab112994 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE. The basic steps:used serum free media to grow the cellsand then use ammonium sulfate precipitation method to purify the antibody which ultimately confirm purity by SDSPAGE,

    I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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