Anti-ENO1 antibody (ab85086)

Rabbit polyclonal ENO1 antibody. Validated in WB, IHC, ICC/IF and tested in Human. Cited in 5 publication(s). Immunogen corresponding to synthetic peptide.

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab85086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
WB 1/200 - 1/1000. Predicted molecular weight: 47 kDa.
IHC-P 1/100.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities

    Belongs to the enolase family.
  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2 phospho D glycerate hydro lyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Alpha enolase antibody
    • Alpha enolase like 1 antibody
    • Alpha-enolase antibody
    • C myc promoter binding protein antibody
    • C-myc promoter-binding protein antibody
    • EC 4.2.1.11 antibody
    • eno1 antibody
    • ENO1L1 antibody
    • ENOA_HUMAN antibody
    • Enolase 1 (alpha) antibody
    • Enolase 1 (alpha) like 1 antibody
    • Enolase 1 antibody
    • Enolase alpha antibody
    • MBP 1 antibody
    • MBP-1 antibody
    • MBP1 antibody
    • MBPB1 antibody
    • MPB 1 antibody
    • MPB-1 antibody
    • MPB1 antibody
    • MYC promoter binding protein 1 antibody
    • NNE antibody
    • Non neural enolase antibody
    • Non-neural enolase antibody
    • Phosphopyruvate hydratase antibody
    • Plasminogen binding protein antibody
    • Plasminogen-binding protein antibody
    • PPH antibody
    • Tau crystallin antibody
    see all

Images

  • All lanes : Anti-ENO1 antibody (ab85086) at 1/200 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : Jurkat cell lysate

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • ab85086, at a 1/100 dilution, staining ENO1 in the cytoplasm and membrane of formalin fixed, paraffin embedded renal tubules by Immunohistochemistry.
  • ICC/IF image of ab85086 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85086, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Sun L  et al. Over-Expression of Alpha-Enolase as a Prognostic Biomarker in Patients with Pancreatic Cancer. Int J Med Sci 14:655-661 (2017). Read more (PubMed: 28824297) »
  • Saxena R  et al. Proteomic profiling of SupT1 cells reveal modulation of host proteins by HIV-1 Nef variants. PLoS One 10:e0122994 (2015). WB, IF ; Human . Read more (PubMed: 25874870) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us.

The antibody ab85086 has been tested in indirect ELISA, and unfortunately we do not have any information regarding its use in sandwich assays.

We have not either mapped the epitope each of the antibodies anti ENO1 available recognise, so it is difficult to predict whether the possible antibody pairs could be used together.

I will check with my colleagues the availability of the antibodies from the ELISA kit, and get back to you as soon as they reply.

Have a very nice day.

Read More

Answer

Thanks for your reply. I apologize for all the trouble with this antibody.
I am sending a free of charge replacement vial of ab71433 on the order *** to the same address as the original order.
Ab71433 is out of stock but we expect the order to be delivered to you last next week.
Please keep me updated about how the replacement antibody works in your Western blot, and let me know if there is anything else that we can do for you.

Read More

Answer

Thank you for your reply and for sending the images of your Western blots.
As alpha enolase is known to form dimers with itself and with other enolase forms, it is possible that the dimers are not reduced which would result in band at approximately twice the expected molecular weight (i.e. 100 kDa instead of around 50 kDa). I would suggest boiling the samples in reducing sample buffer for 10 minutes to make sure the dimers are disrupted. If the bands are still showing up at 100 kDa, then they are probably non-specific bands.
We do guarantee this antibody to work in your assay, so if you would like, I can send a replacement antibody or alternatively issue a credit or refund for your original purchase.
I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you. Enjoy your weekend!

Read More

Answer

Thank you for contacting us and letting us know about the issue with ab85086.
I am sorry but I couldn't open the attachments of the images that were sent with your email. Could you try to send them as a different file type, if possible?
I do have a couple of additional questions, so that I can better understand the situation:
1) What kind of samples are you using?
2) Was the Western blot run in standard reduced and denatured conditions?
3) What molecular weights are the extra bands?
4) What kind of blocking solution was used (milk or BSA)?
I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up