- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-ENO1 antibody
See all ENO1 primary antibodies -
DescriptionRabbit polyclonal to ENO1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Rat, Guinea pig, Zebrafish
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Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 199-412 of Human ENO1 (NP_001419).
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Positive control
- A431 and H1299 whole cell lysates; HeLa cells; 293T, HepG2, Molt-4 and Raji cell lines.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 10% Glycerol, 1.21% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab97586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 47 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
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FunctionMultifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. -
Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
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PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
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Sequence similaritiesBelongs to the enolase family.
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Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
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Post-translational
modificationsISGylated. -
Cellular localizationNucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
- Information by UniProt
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Database links
- Entrez Gene: 2023 Human
- Entrez Gene: 24333 Rat
- Omim: 172430 Human
- SwissProt: P06733 Human
- SwissProt: P04764 Rat
- Unigene: 517145 Human
- Unigene: 4236 Rat
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Alternative names
- 2 phospho D glycerate hydro lyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha enolase antibody
see all
Images
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Western blot - Anti-ENO1 antibody (ab97586)All lanes : Anti-ENO1 antibody (ab97586) at 1/3000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody (ab97586)ab97586, at 1/200 dilution, staining ENO1 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is costained with Hoechst 33342.
References
ab97586 has not yet been referenced specifically in any publications.
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