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    eno1-antibody-ab97586.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-ENO1 antibody (ab97586)

  • Datasheet
  • SDS
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Western blot - Anti-ENO1 antibody (ab97586)
  • Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody (ab97586)

Key features and details

  • Rabbit polyclonal to ENO1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant human ENO1 protein (Active) (ab89248)

View more associated products

Overview

  • Product name

    Anti-ENO1 antibody
    See all ENO1 primary antibodies
  • Description

    Rabbit polyclonal to ENO1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 199-412 of Human ENO1 (NP_001419).

  • Positive control

    • A431 and H1299 whole cell lysates; HeLa cells; 293T, HepG2, Molt-4 and Raji cell lines.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant human ENO1 protein (Active) (ab89248)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Rat
Guinea pig
Zebrafish
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
ICC/IF
1/100 - 1/200.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
ICC/IF
1/100 - 1/200.

Target

  • Function

    Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities

    Belongs to the enolase family.
  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Target information above from: UniProt accession P06733 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2023 Human
    • Entrez Gene: 24333 Rat
    • Omim: 172430 Human
    • SwissProt: P06733 Human
    • SwissProt: P04764 Rat
    • Unigene: 517145 Human
    • Unigene: 4236 Rat
    • Alternative names

      • 2 phospho D glycerate hydro lyase antibody
      • 2-phospho-D-glycerate hydro-lyase antibody
      • Alpha enolase antibody
      • Alpha enolase like 1 antibody
      • Alpha-enolase antibody
      • C myc promoter binding protein antibody
      • C-myc promoter-binding protein antibody
      • EC 4.2.1.11 antibody
      • eno1 antibody
      • ENO1L1 antibody
      • ENOA_HUMAN antibody
      • Enolase 1 (alpha) antibody
      • Enolase 1 (alpha) like 1 antibody
      • Enolase 1 antibody
      • Enolase alpha antibody
      • MBP 1 antibody
      • MBP-1 antibody
      • MBP1 antibody
      • MBPB1 antibody
      • MPB 1 antibody
      • MPB-1 antibody
      • MPB1 antibody
      • MYC promoter binding protein 1 antibody
      • NNE antibody
      • Non neural enolase antibody
      • Non-neural enolase antibody
      • Phosphopyruvate hydratase antibody
      • Plasminogen binding protein antibody
      • Plasminogen-binding protein antibody
      • PPH antibody
      • Tau crystallin antibody
      see all

    Images

    • Western blot - Anti-ENO1 antibody (ab97586)
      Western blot - Anti-ENO1 antibody (ab97586)
      All lanes : Anti-ENO1 antibody (ab97586) at 1/3000 dilution

      Lane 1 : A431 whole cell lysate
      Lane 2 : H1299 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 47 kDa



      10% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody (ab97586)
      Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody (ab97586)
      ab97586, at 1/200 dilution, staining ENO1 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is costained with Hoechst 33342.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab97586? Please let us know so that we can cite the reference in this datasheet.

    ab97586 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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