Anti-ENO1 antibody [EPR10863(B)] (ab155102)
- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-ENO1 antibody [EPR10863(B)]
See all ENO1 primary antibodies -
DescriptionRabbit monoclonal [EPR10863(B)] to ENO1
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, WB, ICC/IF, IPmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
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Positive control
- Purchase matching WB positive control:Recombinant Human ENO1 protein
- WB: MCF7, Jurkat, A431, C2C12, NIH/3T3 and HeLa cell lysates, mouse heart and rat brain tissue lysate. ICC/IF: MCF7 cells. IP: HeLa whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR10863(B)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Rat brain normal tissue lysate - total protein (ab29475)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- MCF7 whole cell lysate (ab3871)
- NIH 3T3 whole cell lysate (ab7179)
- C2C12 whole cell lysate (ab7182)
- Mouse heart tissue lysate - total protein (0 days) (ab7193)
- Mouse heart tissue lysate - total protein (14 days) (ab7194)
- Mouse heart tissue lysate - total protein (7 days) (ab7196)
- Jurkat whole cell lysate (ab7899)
- A431 whole cell lysate (ab7909)
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.Can be blocked with Human ENO1 peptide (ab226769). | |
| ICC/IF | 1/50 - 1/250. | |
| IP | 1/10 - 1/100. |
Target
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FunctionMultifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. -
Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
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PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
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Sequence similaritiesBelongs to the enolase family.
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Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
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Post-translational
modificationsISGylated. -
Cellular localizationNucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
- Information by UniProt
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Database links
- Entrez Gene: 2023 Human
- Entrez Gene: 100045967 Mouse
- Entrez Gene: 100503183 Mouse
- Entrez Gene: 13806 Mouse
- Entrez Gene: 433182 Mouse
- Entrez Gene: 24333 Rat
- Omim: 172430 Human
- SwissProt: P06733 Human
see all -
Alternative names
- 2 phospho D glycerate hydro lyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha enolase antibody
see all
Images
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![Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)](https://www.abcam.com/ps/products/155/ab155102/Images/ab155102-7-ab155102WB3.jpg)
Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution (purified) + Rat brain tissue lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR10863(B)] (ab155102)](https://www.abcam.com/ps/products/155/ab155102/Images/ab155102-4-ab155102ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR10863(B)] (ab155102)Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling ENO1 with purified ab155102 at 1/60. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/60) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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![Flow Cytometry - Anti-ENO1 antibody [EPR10863(B)] (ab155102)](https://www.abcam.com/ps/products/155/ab155102/Images/ab155102-270667-ab155102FC1.jpg)
Flow Cytometry - Anti-ENO1 antibody [EPR10863(B)] (ab155102)Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling ENO1 with purified ab155102 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)](https://www.abcam.com/ps/products/155/ab155102/Images/ab155102-6-ab155102WB2.jpg)
Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)All lanes : Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution (purified)
Lane 1 : C2C12 whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate
Lane 3 : Mouse heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR10863(B)] (ab155102)](https://www.abcam.com/ps/products/155/ab155102/Images/ENO1-Primary-antibodies-ab155102-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR10863(B)] (ab155102)Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling ENO1 with unpurified ab155102 at a dilution of 1/100.
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![Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)](https://www.abcam.com/ps/products/155/ab155102/Images/ab155102-5-ab155102WB1.jpg)
Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)All lanes : Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution (purified)
Lane 1 : MCF-7 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : A431 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-ENO1 antibody [EPR10863(B)] (ab155102)](https://www.abcam.com/ps/products/155/ab155102/Images/ab155102-3-ab155102IP1.jpg)
Immunoprecipitation - Anti-ENO1 antibody [EPR10863(B)] (ab155102)ab155102 (purified) at 1/20 immunoprecipitating ENO1 in HeLa whole cell lysate.
Lane 1 (input): HeLa whole cell lysate (10µg)
Lane 2 (+): ab155102 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab155102 in HeLa whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)](https://www.abcam.com/ps/products/155/ab155102/Images/ENO1-Primary-antibodies-ab155102-1.jpg)
Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)All lanes : Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/1000 dilution (unpurified)
Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 47 kDa
Protocols
References
This product has been referenced in:
- Kovary KM et al. Expression variation and covariation impair analog and enable binary signaling control. Mol Syst Biol 14:e7997 (2018). Read more (PubMed: 29759982) »
- Qi W et al. Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction. Nat Med 23:753-762 (2017). Read more (PubMed: 28436957) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"