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Signal Transduction Metabolism Energy Metabolism
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RecombinantRabMAb

Recombinant Anti-ENO1 antibody [EPR10863(B)] (ab155102)

  • Datasheet
  • SDS
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Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
  • Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
  • Flow Cytometry - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
  • Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
  • Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
  • Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
  • Immunoprecipitation - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
  • Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
  • Anti-ENO1 antibody [EPR10863(B)] (ab155102)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10863(B)] to ENO1
  • Suitable for: Flow Cyt, WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-ENO1 antibody [EPR10863(B)]
    See all ENO1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10863(B)] to ENO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ENO1 aa 1-100 (N terminal). The exact sequence is proprietary. Synthetic peptide within Human ENO1 aa 1-50
    Database link: P06733
    (Peptide available as ab226769)

  • Positive control

    • Purchase matching WB positive control:Recombinant human ENO1 protein (Active)
    • WB: MCF7, Jurkat, A431, C2C12, NIH/3T3 and HeLa whole cell lysate (ab150035), mouse heart and rat brain tissue lysate. ICC/IF: MCF7 cells. IP: HeLa whole cell lysate (ab150035).
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10863(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-ENO1 antibody [EPR10863(B)] (ab205871)
    • Alexa Fluor® 647 Anti-ENO1 antibody [EPR10863(B)] (ab205872)
    • Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)
    • HRP Anti-ENO1 antibody [EPR10863(B)] (ab206272)
  • Assay kits

    • ENO1 Assay Kit (alpha Enolase, Human) (ab117994)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • A431 whole cell lysate (ab30132)
    • NIH 3T3 whole cell lysate (ab7179)
    • C2C12 whole cell lysate (ab7182)
    • Mouse heart tissue lysate - total protein (0 days) (ab7193)
    • Mouse heart tissue lysate - total protein (14 days) (ab7194)
    • Mouse heart tissue lysate - total protein (7 days) (ab7196)
    • Jurkat whole cell lysate (ab7899)
    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant human ENO1 protein (Active) (ab89248)
  • Related Products

    • Recombinant human ENO1 protein (Active) (ab89248)

Applications

Our Abpromise guarantee covers the use of ab155102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
ICC/IF 1/50 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
      MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
    • Tissue specificity

      The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
    • Sequence similarities

      Belongs to the enolase family.
    • Developmental stage

      During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
    • Post-translational
      modifications

      ISGylated.
    • Cellular localization

      Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
    • Target information above from: UniProt accession P06733 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2023 Human
      • Entrez Gene: 100045967 Mouse
      • Entrez Gene: 100503183 Mouse
      • Entrez Gene: 13806 Mouse
      • Entrez Gene: 433182 Mouse
      • Entrez Gene: 24333 Rat
      • Omim: 172430 Human
      • SwissProt: P06733 Human
      • SwissProt: P17182 Mouse
      • SwissProt: P04764 Rat
      • Unigene: 517145 Human
      • Unigene: 372357 Mouse
      • Unigene: 372389 Mouse
      • Unigene: 70666 Mouse
      • Unigene: 4236 Rat
      see all
    • Alternative names

      • 2 phospho D glycerate hydro lyase antibody
      • 2-phospho-D-glycerate hydro-lyase antibody
      • Alpha enolase antibody
      • Alpha enolase like 1 antibody
      • Alpha-enolase antibody
      • C myc promoter binding protein antibody
      • C-myc promoter-binding protein antibody
      • EC 4.2.1.11 antibody
      • eno1 antibody
      • ENO1L1 antibody
      • ENOA_HUMAN antibody
      • Enolase 1 (alpha) antibody
      • Enolase 1 (alpha) like 1 antibody
      • Enolase 1 antibody
      • Enolase alpha antibody
      • MBP 1 antibody
      • MBP-1 antibody
      • MBP1 antibody
      • MBPB1 antibody
      • MPB 1 antibody
      • MPB-1 antibody
      • MPB1 antibody
      • MYC promoter binding protein 1 antibody
      • NNE antibody
      • Non neural enolase antibody
      • Non-neural enolase antibody
      • Phosphopyruvate hydratase antibody
      • Plasminogen binding protein antibody
      • Plasminogen-binding protein antibody
      • PPH antibody
      • Tau crystallin antibody
      see all

    Images

    • Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution (purified) + Rat brain tissue lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 47 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR10863(B)] (ab155102)

      Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling ENO1 with purified ab155102 at 1/60. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/60) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • Flow Cytometry - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      Flow Cytometry - Anti-ENO1 antibody [EPR10863(B)] (ab155102)

      Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling ENO1 with purified ab155102 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      All lanes : Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution (purified)

      Lane 1 : C2C12 whole cell lysate
      Lane 2 : NIH/3T3 whole cell lysate
      Lane 3 : Mouse heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 47 kDa
      Observed band size: 47 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR10863(B)] (ab155102)

      Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling ENO1 with unpurified ab155102 at a dilution of 1/100.

    • Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      All lanes : Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution (purified)

      Lane 1 : MCF-7 whole cell lysate
      Lane 2 : Jurkat whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : A431 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 47 kDa
      Observed band size: 47 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      Immunoprecipitation - Anti-ENO1 antibody [EPR10863(B)] (ab155102)

      ab155102 (purified) at 1/20 immunoprecipitating ENO1 in HeLa whole cell lysate.

      Lane 1 (input): HeLa whole cell lysate (10µg)

      Lane 2 (+): ab155102 + HeLa whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab155102 in HeLa whole cell lysate.

      For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      All lanes : Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/1000 dilution (unpurified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 47 kDa

    • Anti-ENO1 antibody [EPR10863(B)] (ab155102)
      Anti-ENO1 antibody [EPR10863(B)] (ab155102)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (18)

    Publishing research using ab155102? Please let us know so that we can cite the reference in this datasheet.

    ab155102 has been referenced in 18 publications.

    • Wei S  et al. Artesunate inhibits the mevalonate pathway and promotes glioma cell senescence. J Cell Mol Med 24:276-284 (2020). PubMed: 31746143
    • Sheikh MH  et al. Immuno-metabolic impact of the multiple sclerosis patients' sera on endothelial cells of the blood-brain barrier. J Neuroinflammation 17:153 (2020). PubMed: 32386505
    • Yang D  et al. Oxidized ATM promotes breast cancer stem cell enrichment through energy metabolism reprogram-mediated acetyl-CoA accumulation. Cell Death Dis 11:508 (2020). PubMed: 32641713
    • Chen Y  et al. Thermal stress induces glycolytic beige fat formation via a myogenic state. Nature 565:180-185 (2019). PubMed: 30568302
    • Qiao H  et al. Enolase1 overexpression regulates the growth of gastric cancer cells and predicts poor survival. J Cell Biochem 120:18714-18723 (2019). PubMed: 31218757
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Flow Cytometry abreview for Anti-ENO1 antibody [EPR10863(B)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Lymphocytes)
    Permeabilization
    Yes - Commercial reagent
    Gating Strategy
    Live, lymphocytes, CD8+, CD44 high
    Specification
    Lymphocytes
    Preparation
    Cell harvesting/tissue preparation method: Harvested spleen from B6 mouse, stimulated with antii-CD + anti-CD28 in vitro for two days, and performed intracellular staining for flow cytometry.
    Sample buffer: 1xPBS, T cell media (supplemented RPMI, for culture)
    Fixation
    Commercial fixative
    Read More

    Lelisa Gemta

    Verified customer

    Submitted Jun 30 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ENO1 antibody [EPR10863(B)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (D3 mouse embryonic stem cells)
    Permeabilization
    Yes - 0.1% Triton
    Specification
    D3 mouse embryonic stem cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 15°C
    Fixative
    Paraformaldehyde
    Read More

    Miss. Fabiana Luise

    Verified customer

    Submitted Oct 20 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ENO1 antibody [EPR10863(B)]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Sample
    Human Tissue sections (Intracranial xenograft of human glioma line with E)
    Specification
    Intracranial xenograft of human glioma line with E
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Dr. Adriano Cliff

    Verified customer

    Submitted Jan 23 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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