Recombinant
RabMAb

Anti-ENO1 antibody [EPR10863(B)] (ab155102)

Overview

  • Product name
    Anti-ENO1 antibody [EPR10863(B)]
    See all ENO1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10863(B)] to ENO1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ENO1 aa 1-100 (N terminal). The exact sequence is proprietary. Synthetic peptide within Human ENO1 aa 1-50
    Database link: P06733
    (Peptide available as ab226769)

  • Positive control
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab155102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.Can be blocked with Human ENO1 peptide (ab226769).
ICC/IF 1/50 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
      MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
    • Tissue specificity
      The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
    • Pathway
      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
    • Sequence similarities
      Belongs to the enolase family.
    • Developmental stage
      During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
    • Post-translational
      modifications
      ISGylated.
    • Cellular localization
      Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2 phospho D glycerate hydro lyase antibody
      • 2-phospho-D-glycerate hydro-lyase antibody
      • Alpha enolase antibody
      • Alpha enolase like 1 antibody
      • Alpha-enolase antibody
      • C myc promoter binding protein antibody
      • C-myc promoter-binding protein antibody
      • EC 4.2.1.11 antibody
      • eno1 antibody
      • ENO1L1 antibody
      • ENOA_HUMAN antibody
      • Enolase 1 (alpha) antibody
      • Enolase 1 (alpha) like 1 antibody
      • Enolase 1 antibody
      • Enolase alpha antibody
      • MBP 1 antibody
      • MBP-1 antibody
      • MBP1 antibody
      • MBPB1 antibody
      • MPB 1 antibody
      • MPB-1 antibody
      • MPB1 antibody
      • MYC promoter binding protein 1 antibody
      • NNE antibody
      • Non neural enolase antibody
      • Non-neural enolase antibody
      • Phosphopyruvate hydratase antibody
      • Plasminogen binding protein antibody
      • Plasminogen-binding protein antibody
      • PPH antibody
      • Tau crystallin antibody
      see all

    Images

    • Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution (purified) + Rat brain tissue lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 47 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling ENO1 with purified ab155102 at 1/60. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/60) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling ENO1 with purified ab155102 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • All lanes : Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution (purified)

      Lane 1 : C2C12 whole cell lysate
      Lane 2 : NIH/3T3 whole cell lysate
      Lane 3 : Mouse heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 47 kDa
      Observed band size: 47 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling ENO1 with unpurified ab155102 at a dilution of 1/100.

    • All lanes : Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution (purified)

      Lane 1 : MCF-7 whole cell lysate
      Lane 2 : Jurkat whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : A431 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 47 kDa
      Observed band size: 47 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • ab155102 (purified) at 1/20 immunoprecipitating ENO1 in HeLa whole cell lysate.

      Lane 1 (input): HeLa whole cell lysate (10µg)

      Lane 2 (+): ab155102 + HeLa whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab155102 in HeLa whole cell lysate.

      For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/1000 dilution (unpurified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 47 kDa

    References

    This product has been referenced in:
    • Kovary KM  et al. Expression variation and covariation impair analog and enable binary signaling control. Mol Syst Biol 14:e7997 (2018). Read more (PubMed: 29759982) »
    • Qi W  et al. Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction. Nat Med 23:753-762 (2017). Read more (PubMed: 28436957) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    Application
    Flow Cytometry
    Sample
    Mouse Cell (Lymphocytes)
    Permeabilization
    Yes - Commercial reagent
    Gating Strategy
    Live, lymphocytes, CD8+, CD44 high
    Specification
    Lymphocytes
    Preparation
    Cell harvesting/tissue preparation method: Harvested spleen from B6 mouse, stimulated with antii-CD + anti-CD28 in vitro for two days, and performed intracellular staining for flow cytometry.
    Sample buffer: 1xPBS, T cell media (supplemented RPMI, for culture)
    Fixation
    Commercial fixative
    Username

    Lelisa Gemta

    Verified customer

    Submitted Jun 30 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (D3 mouse embryonic stem cells)
    Permeabilization
    Yes - 0.1% Triton
    Specification
    D3 mouse embryonic stem cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 15°C
    Fixative
    Paraformaldehyde
    Username

    Miss. Fabiana Luise

    Verified customer

    Submitted Oct 20 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Sample
    Human Tissue sections (Intracranial xenograft of human glioma line with E)
    Specification
    Intracranial xenograft of human glioma line with E
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Dr. Adriano Cliff

    Verified customer

    Submitted Jan 23 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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