Recombinant
RabMAb

Recombinant Anti-ENO1 antibody [EPR10863(B)] (Alexa Fluor® 647) (ab205872)

Overview

  • Product name

    Anti-ENO1 antibody [EPR10863(B)] (Alexa Fluor® 647)
    See all ENO1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10863(B)] to ENO1 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ENO1. The exact sequence is proprietary.
    Database link: P06733

  • Positive control

    • ICC/IF: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab205872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).

Target

  • Function

    Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities

    Belongs to the enolase family.
  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2 phospho D glycerate hydro lyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Alpha enolase antibody
    • Alpha enolase like 1 antibody
    • Alpha-enolase antibody
    • C myc promoter binding protein antibody
    • C-myc promoter-binding protein antibody
    • EC 4.2.1.11 antibody
    • eno1 antibody
    • ENO1L1 antibody
    • ENOA_HUMAN antibody
    • Enolase 1 (alpha) antibody
    • Enolase 1 (alpha) like 1 antibody
    • Enolase 1 antibody
    • Enolase alpha antibody
    • MBP 1 antibody
    • MBP-1 antibody
    • MBP1 antibody
    • MBPB1 antibody
    • MPB 1 antibody
    • MPB-1 antibody
    • MPB1 antibody
    • MYC promoter binding protein 1 antibody
    • NNE antibody
    • Non neural enolase antibody
    • Non-neural enolase antibody
    • Phosphopyruvate hydratase antibody
    • Plasminogen binding protein antibody
    • Plasminogen-binding protein antibody
    • PPH antibody
    • Tau crystallin antibody
    see all

Images

  • ab205872 staining ENO1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205872 at a 1/250 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab205872 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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