Overview

  • Product name

    Anti-ENO1 antibody [EPR10864(B)]
    See all ENO1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10864(B)] to ENO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human ENO1.

  • Positive control

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab155955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
      MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
    • Tissue specificity

      The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
    • Sequence similarities

      Belongs to the enolase family.
    • Developmental stage

      During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
    • Post-translational
      modifications

      ISGylated.
    • Cellular localization

      Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2 phospho D glycerate hydro lyase antibody
      • 2-phospho-D-glycerate hydro-lyase antibody
      • Alpha enolase antibody
      • Alpha enolase like 1 antibody
      • Alpha-enolase antibody
      • C myc promoter binding protein antibody
      • C-myc promoter-binding protein antibody
      • EC 4.2.1.11 antibody
      • eno1 antibody
      • ENO1L1 antibody
      • ENOA_HUMAN antibody
      • Enolase 1 (alpha) antibody
      • Enolase 1 (alpha) like 1 antibody
      • Enolase 1 antibody
      • Enolase alpha antibody
      • MBP 1 antibody
      • MBP-1 antibody
      • MBP1 antibody
      • MBPB1 antibody
      • MPB 1 antibody
      • MPB-1 antibody
      • MPB1 antibody
      • MYC promoter binding protein 1 antibody
      • NNE antibody
      • Non neural enolase antibody
      • Non-neural enolase antibody
      • Phosphopyruvate hydratase antibody
      • Plasminogen binding protein antibody
      • Plasminogen-binding protein antibody
      • PPH antibody
      • Tau crystallin antibody
      see all

    Images

    • All lanes : Anti-ENO1 antibody [EPR10864(B)] (ab155955) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 47 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ENO1 with ab155955 at 1/100 dilution.

    References

    This product has been referenced in:

    • Jain S  et al. Salivary proteome patterns of individuals exposed to High Altitude. Arch Oral Biol 96:104-112 (2018). Read more (PubMed: 30219637) »
    • Sun L  et al. Alpha-enolase promotes gastric cancer cell proliferation and metastasis via regulating AKT signaling pathway. Eur J Pharmacol 845:8-15 (2018). Read more (PubMed: 30582908) »
    See all 3 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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