Key features and details
- Rabbit polyclonal to ENO1 (HRP)
- Suitable for: WB
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG
Product nameAnti-ENO1 antibody (HRP)
See all ENO1 primary antibodies
DescriptionRabbit polyclonal to ENO1 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Chicken, Cow, Human, Orangutan
Recombinant full length protein corresponding to Mouse ENO1 aa 2-433.
Database link: P17182
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notes>95%,Protein G purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab193129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 48 kDa.|
FunctionMultifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Sequence similaritiesBelongs to the enolase family.
Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
Cellular localizationNucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
- Information by UniProt
- 2 phospho D glycerate hydro lyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha enolase antibody
All lanes : Anti-ENO1 antibody (HRP) (ab193129) at 0.42 µg/ml
Lane 1 : PC3
Lane 2 : HepG2 whole cell lysate
Lane 3 : NIH/3T3 whole cell lysate
Lane 4 : Rat heart
Lane 5 : Rat brain
Lane 6 : Rat skeletal muscle
Lane 7 : Mouse kidney tissue lysate
Lane 8 : Mouse liver tissue lysate
Predicted band size: 48 kDa
Observed band size: 34,44,48 kDa why is the actual band size different from the predicted?
ab193129 has not yet been referenced specifically in any publications.