ENO1 Assay Kit (alpha Enolase, Human) (ab117994)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture extracts, Tissue Extracts
- Sensitivity: 125 ng/ml
Overview
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Product name
ENO1 Assay Kit (alpha Enolase, Human) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell extract 9 9% Inter-assay Sample n Mean SD CV% Cell extract 4 11% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
125 ng/ml -
Range
125 ng/ml - 2000 ng/ml -
Recovery
90 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 63 % - % Serum 140 % - % -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Product overview
ENO1 Assay Kit (alpha Enolase, Human) ab117994 is used to determine enolase 1 (ENO1) activity in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The enolase activity is determined via a coupled reaction to the consumption of NADH in an assay buffer.
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Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Blocking Buffer 1 x 6ml 20X Buffer 1 x 20ml Base Buffer 1 x 24ml Extraction Buffer (ab260490) 1 x 15ml LDH (Lactate Dehydrogenase) 1 x 187.5 units Microplate 96 antibody coated wells in 12 strips 1 unit PK (Pyruvate Kinase) 1 x 187.5 units Reagent Mix 1 x 1ml -
Research areas
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Database links
- Entrez Gene: 2023 Human
- SwissProt: P06733 Human
Associated products
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Related Products
Images
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Scheme of reactions used in this kit. Abbreviations are as follows: enolase (ENO), 2-phospho-D-glycerate (2PG), phosphoenolpyruvate (PEP), pyruvate kinase (PK), lactate dehydrogenase (LDH).
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Immunofluorescent detection of ENO1 in cytosol in HeLa cells using ab112994.
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Example of dilution series prepared from HepG2 cells extract.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab117994 has been referenced in 6 publications.
- Yang G et al. Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis. EMBO Rep 22:e50967 (2021). PubMed: 33372411
- Zhang W et al. Cinnamaldehyde Enhances Antimelanoma Activity through Covalently Binding ENO1 and Exhibits a Promoting Effect with Dacarbazine. Cancers (Basel) 12:N/A (2020). PubMed: 32013122
- Sutter CH et al. AHR Regulates Metabolic Reprogramming to Promote SIRT1-Dependent Keratinocyte Differentiation. J Invest Dermatol 139:818-826 (2019). PubMed: 30393078
- Huang H et al. p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis. Mol Cell 70:663-678.e6 (2018). PubMed: 29775581
- Marchesini MI et al. A T4SS Effector Targets Host Cell Alpha-Enolase Contributing to Brucella abortus Intracellular Lifestyle. Front Cell Infect Microbiol 6:153 (2016). PubMed: 27900285
- Bottermann K et al. Systematic Analysis Reveals Elongation Factor 2 and a-Enolase as Novel Interaction Partners of AKT2. PLoS One 8:e66045 (2013). PubMed: 23823123