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Signal Transduction Metabolism Energy Metabolism
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Anti-ENO3 antibody (ab126259)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Western blot - Anti-ENO3 antibody (ab126259)
  • Immunocytochemistry/ Immunofluorescence - Anti-ENO3 antibody (ab126259)

Key features and details

  • Rabbit polyclonal to ENO3
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human ENO3 protein (ab113127)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ENO3 antibody
    See all ENO3 primary antibodies
  • Description

    Rabbit polyclonal to ENO3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-371 of Human ENO3.

  • Positive control

    • A549, HeLa, HepG and HCT116 whole cell lysates; HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HepG2 cytoplasmic extract lysate (ab14659)
    • HeLa whole cell lysate (ab29545)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human ENO3 protein (ab113127)

Applications

Our Abpromise guarantee covers the use of ab126259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 47 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Appears to have a function in striated muscle development and regeneration.
  • Tissue specificity

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway

    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Involvement in disease

    Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13) [MIM:612932]. A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected on ultrastructural analysis.
  • Sequence similarities

    Belongs to the enolase family.
  • Developmental stage

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localization

    Cytoplasm. Localized to the Z line. Some colocalization with CKM at M-band.
  • Target information above from: UniProt accession P13929 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2027 Human
    • Omim: 131370 Human
    • SwissProt: P13929 Human
    • Unigene: 224171 Human
    • Alternative names

      • 2 phospho D glycerate hydro lyase antibody
      • 2 phospho D glycerate hydrolyase antibody
      • 2-phospho-D-glycerate hydro-lyase antibody
      • Beta enolase antibody
      • Beta-enolase antibody
      • ENO3 antibody
      • ENOB_HUMAN antibody
      • Enolase 3 (beta muscle) antibody
      • Enolase 3 antibody
      • Enolase3 antibody
      • GSD13 antibody
      • MSE antibody
      • Muscle specific enolase antibody
      • Muscle-specific enolase antibody
      • Skeletal muscle enolase antibody
      see all

    Images

    • Western blot - Anti-ENO3 antibody (ab126259)
      Western blot - Anti-ENO3 antibody (ab126259)
      Anti-ENO3 antibody (ab126259) at 1/10000 dilution + HepG2 cell lysate at 30 µg

      Predicted band size: 47 kDa



      10% SDS-PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-ENO3 antibody (ab126259)
      Immunocytochemistry/ Immunofluorescence - Anti-ENO3 antibody (ab126259)
      ab126259 at 1/500 dilution staining ENO3 in methanol-fixed HeLa cells by Immunofluorescence (green). Alpha tubulin filaments are stained red.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab126259? Please let us know so that we can cite the reference in this datasheet.

    ab126259 has been referenced in 1 publication.

    • Insenser M  et al. A nontargeted study of muscle proteome in severely obese women with androgen excess compared with severely obese men and nonhyperandrogenic women. Eur J Endocrinol 174:389-98 (2016). PubMed: 26671973

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-ENO3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Tissue lysate - whole (skeletal muscle)
    Specification
    skeletal muscle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Rafael Montes Nieto

    Verified customer

    Submitted Oct 31 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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