Anti-ENO3 antibody (ab126259)
- Datasheet
- References
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Overview
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Product name
Anti-ENO3 antibody
See all ENO3 primary antibodies -
Description
Rabbit polyclonal to ENO3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-371 of Human ENO3.
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Positive control
- A549, HeLa, HepG and HCT116 whole cell lysates; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 47 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Appears to have a function in striated muscle development and regeneration. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Involvement in disease
Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13) [MIM:612932]. A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected on ultrastructural analysis. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Cellular localization
Cytoplasm. Localized to the Z line. Some colocalization with CKM at M-band. - Information by UniProt
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Database links
- Entrez Gene: 2027 Human
- Omim: 131370 Human
- SwissProt: P13929 Human
- Unigene: 224171 Human
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Alternative names
- 2 phospho D glycerate hydro lyase antibody
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
see all
Images
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Western blot - Anti-ENO3 antibody (ab126259)Anti-ENO3 antibody (ab126259) at 1/10000 dilution + HepG2 cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ENO3 antibody (ab126259)ab126259 at 1/500 dilution staining ENO3 in methanol-fixed HeLa cells by Immunofluorescence (green). Alpha tubulin filaments are stained red.
References
ab126259 has not yet been referenced specifically in any publications.
