Overview

  • Product name
    Anti-ENO3 antibody
    See all ENO3 primary antibodies
  • Description
    Rabbit polyclonal to ENO3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-371 of Human ENO3.

  • Positive control
    • A549, HeLa, HepG and HCT116 whole cell lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab126259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 47 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Appears to have a function in striated muscle development and regeneration.
  • Tissue specificity
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Involvement in disease
    Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13) [MIM:612932]. A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected on ultrastructural analysis.
  • Sequence similarities
    Belongs to the enolase family.
  • Developmental stage
    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localization
    Cytoplasm. Localized to the Z line. Some colocalization with CKM at M-band.
  • Information by UniProt
  • Database links
    • Alternative names
      • 2 phospho D glycerate hydrol yase antibody
      • 2 phospho D glycerate hydrolyase antibody
      • 2-phospho-D-glycerate hydro-lyase antibody
      • Beta enolase antibody
      • Beta-enolase antibody
      • ENO3 antibody
      • ENOB_HUMAN antibody
      • Enolase 3 (beta muscle) antibody
      • Enolase 3 antibody
      • Enolase3 antibody
      • GSD13 antibody
      • MSE antibody
      • Muscle specific enolase antibody
      • Muscle-specific enolase antibody
      • Skeletal muscle enolase antibody
      see all

    Images

    • Western blot - Anti-ENO3 antibody (ab126259)
      Western blot - Anti-ENO3 antibody (ab126259)
      Anti-ENO3 antibody (ab126259) at 1/10000 dilution + HepG2 cell lysate at 30 µg

      Predicted band size: 47 kDa



      10% SDS-PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-ENO3 antibody (ab126259)
      Immunocytochemistry/ Immunofluorescence - Anti-ENO3 antibody (ab126259)
      ab126259 at 1/500 dilution staining ENO3 in methanol-fixed HeLa cells by Immunofluorescence (green). Alpha tubulin filaments are stained red.

    References

    ab126259 has not yet been referenced specifically in any publications.

    Publishing research using ab126259? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-ENO3 antibody

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Tissue lysate - whole (skeletal muscle)
    Specification
    skeletal muscle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Dr. Rafael Montes Nieto

    Verified customer

    Submitted Oct 31 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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