Anti-ENO3 antibody (ab231121)
Key features and details
- Rabbit polyclonal to ENO3
- Suitable for: WB
- Reacts with: Chicken
- Isotype: IgG
Overview
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Product name
Anti-ENO3 antibody
See all ENO3 primary antibodies -
Description
Rabbit polyclonal to ENO3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Chicken -
Immunogen
Recombinant full length protein corresponding to Chicken ENO3 aa 1 to the C-terminus. Two N-terminal tags, His-tag and GST-tag. Expressed in E. coli.
Database link: P07322 -
Positive control
- WB: Recombinant chicken ENO3 protein. Chicken muscle and brain tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. |
Target
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Function
Appears to have a function in striated muscle development and regeneration. -
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
Involvement in disease
Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13) [MIM:612932]. A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected on ultrastructural analysis. -
Sequence similarities
Belongs to the enolase family. -
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
Cellular localization
Cytoplasm. Localized to the Z line. Some colocalization with CKM at M-band. - Information by UniProt
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Alternative names
- 2 phospho D glycerate hydro lyase antibody
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
see all
Images
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All lanes : Anti-ENO3 antibody (ab231121) at 2 µg/ml
Lane 1 : Chicken muscle lysate
Lane 2 : Chicken brain lysate
Secondary
All lanes : HRP-linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique. -
Anti-ENO3 antibody (ab231121) at 2 µg/ml + Recombinant chicken ENO3 protein
Developed using the ECL technique.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231121 has not yet been referenced specifically in any publications.