RecombinantRabMAb

Recombinant Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)

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Western blot - Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)
  • Western blot - Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)
  • Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11366(B)] to ENO3 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free
    See all ENO3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11366(B)] to ENO3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249337 is the carrier-free version of ab157474. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249337 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab249337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.

    • Target

    • Function

      Appears to have a function in striated muscle development and regeneration.

    • Tissue specificity

      The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

    • Involvement in disease

      Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13) [MIM:612932]. A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected on ultrastructural analysis.

    • Sequence similarities

      Belongs to the enolase family.

    • Developmental stage

      During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

    • Cellular localization

      Cytoplasm. Localized to the Z line. Some colocalization with CKM at M-band.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 2 phospho D glycerate hydro lyase antibody
      • 2 phospho D glycerate hydrolyase antibody
      • 2-phospho-D-glycerate hydro-lyase antibody
      • Beta enolase antibody
      • Beta-enolase antibody
      • ENO3 antibody
      • ENOB_HUMAN antibody
      • Enolase 3 (beta muscle) antibody
      • Enolase 3 antibody
      • Enolase3 antibody
      • GSD13 antibody
      • MSE antibody
      • Muscle specific enolase antibody
      • Muscle-specific enolase antibody
      • Skeletal muscle enolase antibody
      see all

    Images

    • Western blot - Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)
      Western blot - Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)
      All lanes : Anti-ENO3 antibody [EPR11366(B)] (ab157474) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Human spleen lysate
      Lane 4 : Fetal heart lysate
      Lane 5 : Fetal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 47 kDa



      This data was developed using ab157474, the same antibody clone in a different buffer formulation.

    • Western blot - Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)
      Western blot - Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)
      Anti-ENO3 antibody [EPR11366(B)] (ab157474) at 1/1000 dilution + immunoprecipitation pellet from fetal muscle lysate

      Secondary
      HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG

      Predicted band size: 47 kDa



      This data was developed using ab157474, the same antibody clone in a different buffer formulation.

      Note: primary antibody diluted with 1% BSA.

    • Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)
      Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab249337? Please let us know so that we can cite the reference in this datasheet.

    ab249337 has not yet been referenced specifically in any publications.

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