Key features and details
- Rabbit polyclonal to Enolase
- Suitable for: WB
- Reacts with: Staphylococcus aureus
- Isotype: IgG
Product nameAnti-Enolase antibody
DescriptionRabbit polyclonal to Enolase
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Staphylococcus aureus
Recombinant full length protein corresponding to Staphylococcus aureus Enolase aa 1-434.
MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVE LRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALD GTPNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMN IVNGGSHSDAPIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGL ETAVGDEGGFAPKFEGTEDAVETIIQAIEAAGYKPGEEVFLGFDCASSEF YENGVYDYSKFEGEHGAKRTAAEQVDYLEQLVDKYPIITIEDGMDENDWD GWKQLTERIGDRVQLVGDDLFVTNTEILAKGIENGIGNSILIKVNQIGTL TETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSR TDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK
Database link: P64078
- WB: Recombinant Staphylococcus aureus Enolase protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab229919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 64 kDa (predicted molecular weight: 47 kDa).|
All lanes : Anti-Enolase antibody (ab229919) at 1/500 dilution
Lane 1 : Recombinant Staphylococcus aureus Enolase protein at 0.06 µg
Lane 2 : Recombinant Staphylococcus aureus Enolase protein at 0.03 µg
Lane 3 : Recombinant Staphylococcus aureus Enolase protein at 0.015 µg
Lane 4 : Recombinant Staphylococcus aureus Enolase protein at 0.0075 µg
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229919 has not yet been referenced specifically in any publications.