Key features and details
- Detection method: Colorimetric/Fluorometric
- Sample type: Adherent cells, Cell culture supernatant, Suspension cells, Tissue
Product nameEnolase Assay Kit
See all ENO1 kits
Sample typeCell culture supernatant, Tissue, Adherent cells, Suspension cells
In the Enolase Assay Kit (ab241024) enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with the probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm).
This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Enolase Assay Buffer 1 x 25ml Enolase Converter (Lyophilized) 1 vial Enolase Developer (Lyophilized) 1 vial Enolase Positive Control (Lyophilized) 1 vial Enolase Substrate Mix (Lyophilized) 1 vial H2O2 Standard (0.88 M) 1 x 0.1ml Probe (in DMSO) 1 x 0.2ml
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
FunctionMultifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Sequence similaritiesBelongs to the enolase family.
Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
Cellular localizationNucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
- Information by UniProt
- 2 phospho D glycerate hydro lyase
- 2-phospho-D-glycerate hydro-lyase
- Alpha enolase
ab241024 has not yet been referenced specifically in any publications.