Recombinant Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109)
![Western blot - Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109)](https://www.abcam.com/ps/products/282/ab282109/Images/ab282109-1-Mouse-monoclonal-3eNOSNOSTypeIII-to-eNOS-western-blot-huvec.jpg "Western blot - Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [3/eNOS/NOS Type III] to eNOS
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-eNOS antibody [3/eNOS/NOS Type III]
See all eNOS primary antibodies -
Description
Mouse monoclonal [3/eNOS/NOS Type III] to eNOS -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Human spleen, Mouse spleen tissues. WB: HUVEC, Ea.Hy926, bEnd.3 whole cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3/eNOS/NOS Type III -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab282109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/2000.
|
| Notes |
|---|
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/2000. |
Target
-
Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. -
Tissue specificity
Platelets, placenta, liver and kidney. -
Involvement in disease
Variation in NOS3 seem to be associated with susceptibility to coronary spasm. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
Post-translational
modificationsPhosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. -
Cellular localization
Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity. - Information by UniProt
-
Database links
- Entrez Gene: 4846 Human
- Entrez Gene: 18127 Mouse
- Omim: 163729 Human
- SwissProt: P29474 Human
- SwissProt: P70313 Mouse
- Unigene: 647092 Human
- Unigene: 258415 Mouse
-
Alternative names
- cNOS antibody
- Constitutive NOS antibody
- EC NOS antibody
see all
Images
-
Western blot - Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109)All lanes : Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109) at 1/2000 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : Ea.Hy926 (Human somatic cell hybrid endothelial), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates should be made freshly and used in WB immediately to minimize protein degradation.
Negative control: HeLa (PMID:19559671).
Exposure time: 37 seconds.
-
![Western blot - Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109)](https://www.abcam.com/ps/products/282/ab282109/Images/ab282109-2-Mouse-monoclonal-3eNOSNOSTypeIII-to-eNOS-western-blot-bend3.jpg)
Western blot - Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109)Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109) at 1/5000 dilution + bEnd.3 (mouse brain endothelioma), whole cell lysate at 10 µg
Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates should be made freshly and used in WB immediately to minimize protein degradation.
Exposure time: 3 seconds.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109)](https://www.abcam.com/ps/products/282/ab282109/Images/ab282109-4-Mouse-monoclonal-3eNOSNOSTypeIII-to-eNOS-human-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling eNOS with ab282109 at 1/2000 dilution (0.518 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in endothelial cells of human spleen. The section was incubated with ab282109 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109)](https://www.abcam.com/ps/products/282/ab282109/Images/ab282109-5-Mouse-monoclonal-3eNOSNOSTypeIII-to-eNOS-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling eNOS with ab282109 at 1/2000 dilution (0.518 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in endothelial cells of mouse spleen. The section was incubated with ab282109 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109)](https://www.abcam.com/ps/products/282/ab282109/Images/ab282109-3-Benefits-of-Recombinant-antibodies.jpg)
Anti-eNOS antibody [3/eNOS/NOS Type III] (ab282109)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab282109 has not yet been referenced specifically in any publications.