Western blot abreview for Anti-eNOS antibody [M221]

Excellent
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (Skeletal muscle)
Loading amount
20 µg
Specification
Skeletal muscle
Gel Running Conditions
Reduced Denaturing (8% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk in TBS-Tween (0.005%)

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Mouse IgG, HRP-linked Antibody
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
20 second(s)
Bands
Specific: 133 kDa

Additional data

Additional Notes
Nitrocellulose membrane;
1% SDS lysis buffer with 1:100 protease inhibitors.

Dr. G.K Sakellariou

Verified customer

Submitted May 15 2013

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