Western blot abreview for Anti-eNOS antibody [M221]

Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (8% Gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Mouse IgG, HRP-linked Antibody
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Bands
Specific: 133 kDa
Exposure
40 second(s)

Additional data

Additional Notes
Representative western blot of nNOS protein from skeletal muscles of Cu ZnSOD knockout and WT mice.
PubMed ID
24378874: View

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Submitted Jan 09 2014

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