Western blot abreview for Anti-eNOS antibody [M221]

Inconclusive
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Tissue lysate - whole (Rat brain extract)
Specification
Rat brain extract
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Anti-mouse HRP
Host species: Horse
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/2000

Detection

Detection method
ECL+
Bands
Specific: n/a kDa Non-specific: 15, 20, 37 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
none
Positive control
Rat brain extract and unactivated human platelet lysate (varying dilutions of platelets originally at 3x10^8 platelets/ml, 1/10-1/100)

Additional data

Additional Notes
Loading control was run using an abcam Anti-GAPDH HRP. A single band was observed at 37kD in the rat brain extract lane (1/5000 dilution, in 5% BSA, 7 sec exposure time).
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.

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Verified customer

Submitted Jun 23 2014

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