Western blot abreview for Anti-eNOS antibody [M221]

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (skeletal muscle (plantaris))
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
40 µg
Specification
skeletal muscle (plantaris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% TBST-milk
Dilution
1/625

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Peroxidase AffiniPure Donkey Anti-Mouse IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
-
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: ~133 kDa Non-specific: - kDa
Positive control
-

Additional data

Additional Notes
PVDF membrane - 0.45 um pore size 6 animals from control group, 20 animals from experimental groups
Abcam response
Thank you for participating in our Abtrial program and your valuable feedback. We are pleased that this antibody worked well for you in rat, a species that has not been validated yet. We welcome any further feedback and Abreviews from other customers who have used this antibody in rat which will help us assess how well the antibody works in this species.

Abcam user community

Verified customer

Submitted Dec 04 2015

Sign up