Western blot abreview for Anti-eNOS antibody [M221]

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-mouse IgG-HRP : sc-2005
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 133 kDa

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Submitted Jan 26 2016

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