Western blot abreview for Anti-eNOS antibody [M221]

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 3% milk (TBS-tween)
Dilution
1/1000

Secondary antibody

Dilution
1/6000
Name
Non-Abcam antibody was used: Goat anti-Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
5 minute(s) and 20 second(s)
Bands
Specific: 135 kDa

Additional data

Additional Notes
Lysis Buffer: Ripa Buffer + Protease inhibitors (1:1000)

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Submitted Feb 09 2016

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