Application
Western blot
Sample
Rat Tissue lysate - other (Heart)
Gel Running Conditions
Reduced Denaturing (NuPAGE 4-12% Bis-Tris)
Loading amount
100 µg
Specification
Heart
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/20
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: Goat anti Mouse926-68070
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Detection
Detection method
Fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 140 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Jan 06 2020