Product nameAnti-eNOS antibody, prediluted
See all eNOS primary antibodies
DescriptionRabbit polyclonal to eNOS, prediluted
Tested applicationsSuitable for: IHC-Glut, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig
- HUVEC cells and placenta.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.6
Preservative: 0.1% Sodium azide
Constituents: 99% Tris buffered saline, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Glut||1/1. PubMed: 19926873|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionProduces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Tissue specificityPlatelets, placenta, liver and kidney.
Involvement in diseaseVariation in NOS3 seem to be associated with susceptibility to coronary spasm.
Sequence similaritiesBelongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
modificationsPhosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
Cellular localizationCell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
- Information by UniProt
- cNOS antibody
- Constitutive NOS antibody
- EC NOS antibody
Human placenta stained with ab15280 in IHC paraffin fixed sections.
Ab15280 (used neat) staining human B-lineage Lymphoma tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 1 hour. Bound antibody was detected using a biotinylated horse anti-mouse and rabbit IgG antibody.
This image is courtesy of an Abreview submitted by Francisca Wu on 10 March 2006.
This product has been referenced in:
- Weyers JJ et al. Effects of cell grafting on coronary remodeling after myocardial infarction. J Am Heart Assoc 2:e000202 (2013). IHC-P ; Mouse . Read more (PubMed: 23723253) »
- Feng Y et al. Brain-selective overexpression of human Angiotensin-converting enzyme type 2 attenuates neurogenic hypertension. Circ Res 106:373-82 (2010). IHC (PFA fixed) ; Mouse . Read more (PubMed: 19926873) »