Immunocytochemistry/ Immunofluorescence abreview for Anti-eNOS (phospho S1177) antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (rat aortic endothelial cells)
Permeabilization
Yes - 0.1% triton X-100
Specification
rat aortic endothelial cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% goat serum in PBS
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Life technologies Alexa 633 Anti Rabbit IgG (H+L)
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor­ 647

Additional data

Additional Notes
we have also submitted an abreview for this antibody with frozen sections. Also for the secondary antibody we used Alexa Fluor 633 which was not included in the list. so I just chose the closest one.

Mozhgan Shojaee

Verified customer

Submitted Aug 04 2017

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